- 著者
- Miyuki Yokoyama Tetsu Watanabe Yoichiro Otaki Ken Watanabe Taku Toshima Takayuki Sugai Tetsuya Takahashi Daisuke Kinoshita Harutoshi Tamura Satoshi Nishiyama Hiroki Takahashi Takanori Arimoto Tetsuro Shishido Sou Yamauchi Tamon Yamanaka Takuya Miyamoto Isao Kubota
- 出版者
- The Japanese Circulation Society
- 雑誌
- Circulation Journal (ISSN:13469843)
- 巻号頁・発行日
- pp.CJ-17-0731, (Released:2017-11-30)
- 参考文献数
- 34
- 被引用文献数
- 39
Background:Peripheral artery disease (PAD) is an athero-occlusive disease and a known risk factor for cardiovascular events. The controlling nutritional status (CONUT) score and geriatric nutritional risk index (GNRI) are objective tools for evaluating malnutrition and are reportedly associated with poor clinical outcomes in patients with fatal diseases. However, the effect of malnutrition on the clinical outcomes in patients with PAD remains unclear.
- 著者
- Taiki Mori Yoshihito Sakai Mitsunori Kayano Akio Matsuda Keisuke Oboki Kenji Matsumoto Atsushi Harada Shumpei Niida Ken Watanabe
- 出版者
- The Japanese Society for Spine Surgery and Related Research
- 雑誌
- Spine Surgery and Related Research (ISSN:2432261X)
- 巻号頁・発行日
- vol.1, no.4, pp.211-217, 2017-10-20 (Released:2017-11-27)
- 参考文献数
- 45
- 被引用文献数
- 13 14
Introduction: Molecular pathways involved in ligamentum flavum (LF) hypertrophy are still unclarified. The purpose of this study was to characterize LF hypertrophy by microRNA (miRNA) profiling according to the classification of lumbar spinal stenosis (LSS).Methods: Classification of patients with LSS into ligamentous and non-ligamentous cases was conducted by clinical observation and the morphometric parameter adopting the LF/spinal canal area ratio (LSAR) from measurements of magnetic resonance imaging (MRI) T2 weighed images. LF from patients with ligamentous stenosis (n=10) were considered as the degenerative hypertrophied samples, and those from patients with non-ligamentous LSS (n=7) and lumbar disc herniation (LDH, n=3) were used as non-hypertrophied controls. Profiling of miRNA from all samples was conducted by Agilent microarray. Microarray data analysis was performed with GeneSpring GX, and pathway analysis was performed using Ingenuity Pathway Analysis.Results: The mean LSAR in the ligamentous group was significantly higher than that in the control group (0.662±0.154 vs 0.301±0.068, p=0.0000171). Ten significantly differentially expressed miRNA were identified and taken as a signature of LF hypertrophy: nine miRNA showed down-regulated expression, and one showed up-regulated expression in the ligamentous LF. Among those, miR-423-5p (rs=-0.473, p<0.05), miR-4306 (rs=-0.628, p<0.01), miR-516b-5p (rs=-0.629, p<0.01), and miR-497-5p (rs=0.461, p<0.05) were correlated to the LSAR. Pathway analysis predicted aryl hydrocarbon receptor signaling (p<0.01), Wnt/β-catenin signaling (p<0.01), and insulin receptor signaling (p<0.05) as canonical pathways associated with the miRNA signature.Conclusions: Classification based on quantification of the MRI axial image is useful for studying hypertrophy of the LF. Aryl hydrocarbon receptor and Wnt/β-catenin signaling may be involved in LF hypertrophy.
- 著者
- Akihiko Nishikimi Masayo Kojima Ken Watanabe Atsushi Watanabe Mikako Yasuoka Hironori Oshima Haruhiko Tokuda Shumpei Niida
- 出版者
- National Center for Global Health and Medicine
- 雑誌
- GHM Open (ISSN:2436293X)
- 巻号頁・発行日
- vol.1, no.1, pp.40-42, 2021-08-29 (Released:2021-09-01)
- 参考文献数
- 9
- 被引用文献数
- 2
To achieve effective prevention and control strategies for COVID-19, regular survey of seroprevalence of antibodies against severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is essential. Using four serological tests, we examined the residual sera collected in an annual medical checkup of the staff members of the National Center for Geriatrics and Gerontology in Aichi Prefecture, Central Japan in June 2020. Of the 631 samples, two were positive for anti-SARS-CoV-2 antibodies in at least two tests, showing a seroprevalence of 0.32%. Four subjects showed positive results in only one test. All individuals were asymptomatic and had not been in close contact with patients diagnosed with COVID-19. Multiple antibody tests could be used to assess the prevalence of SARS-CoV-2 infection including individuals without COVID-19 symptoms.