著者

Shutaro Kobayashi Kazunori O’Hashi Keisuke Kaneko Satomi Kobayashi Shouhei Ogisawa Morio Tonogi Satoshi Fujita Masayuki Kobayashi

出版者

Nihon University School of Dentistry

雑誌

Journal of Oral Science (ISSN:13434934)

巻号頁・発行日

pp.21-0528, (Released:2022-02-16)

参考文献数

28

被引用文献数

1

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Purpose: Unless the phenotype of the transgenic mice is distinguishable, genotyping in each mouse is required prior to experiments. This study aimed to establish a new identification method for the phenotype in Thy1-GCaMP6s transgenic mice to reduce the cost and time.Methods: Tail biopsies (2 mm) were performed under general anesthesia with isoflurane in 3 to 4-week-old mice. Then, the resected tail was cut again with a sharp razor, and the cross-sections were observed with two-photon microscopy (excitation wavelength = 940 nm). The emitted light was split into green and red light by a dichroic mirror (570 nm) with bandpass filters (495-540 nm for green, 575-645 nm for red).Results: Two types of expressed fluorescent pattern were found in the tail tissue: the presence of green fluorescent structures (type 1) and the absence of the structures (type 2). Cortical imaging confirmed that type 1 expressed the cortical GCaMP6s, while type 2 did not.Conclusion: These results suggest that observation of the cross-sectioned tail in Thy1-GCaMP6s mice enabled to identify the phenotype within approximately 10 min/mouse, which reduces the cost and time for genotyping.

著者

ODAWARA O. ANZAWA S. TANJI A. NAKAMURA T. YODA S. SAMEJIMA H.

出版者

日本マイクログラビティ応用学会

雑誌

JASMA : Journal of the Japan Society of Microgravity Application (ISSN:09153616)

巻号頁・発行日

vol.12, no.1, pp.11-18, 1995-02-20

参考文献数

5

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Combustion synthesis technology can be advantageously applied to high-temperature experiments under microgravity environments(MOE) not only as a direct synthesizing method for high-temperature materials but also as a super-heating chemical oven, because it is possi­ble to get high temperatures more rapidly compared to conventional electric furnaces. In the present work, the combustion synthesis of ZrO2-A}zO3-Fe and TiB2-Al-B composite systems have been carried out using a TR-IA sounding rocket which provides about 10- 4 g0 for six minutes. As the former result, more uniform distribution and finer particles of ZrO2 could be attained under MOE, which would be caused from no disturbance in mass migration. As the latter, the composite layered particles composed of TiB2 and Al-B system synthesized under MOE were more uniform compared to those obtained on earth, which would result in the formation of denser cermet-like products.

著者

VALIS O.

雑誌

Scand J Gastroenterol

巻号頁・発行日

vol.28, pp.705-708, 1993

被引用文献数

1 32

著者

Hiroshi Tateishi Mika Tateishi Mohamed O Radwan Takuya Masunaga Kosuke Kawatashiro Yasunori Oba Misato Oyama Natsuki Inoue-Kitahashi Mikako Fujita Yoshinari Okamoto Masami Otsuka

出版者

The Pharmaceutical Society of Japan

雑誌

Chemical and Pharmaceutical Bulletin (ISSN:00092363)

巻号頁・発行日

vol.69, no.11, pp.1123-1130, 2021-11-01 (Released:2021-11-01)

参考文献数

41

被引用文献数

11

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A disintegrin and metalloproteinase 17 (ADAM17) is a zinc-dependent enzyme that catalyzes the cleavage of the extracellular domains of various transmembrane proteins. ADAM17 is regarded as a promising drug target for the suppression of various diseases, including cancer metastasis. We synthesized a new ADAM17 inhibitor, SN-4, composed of a zinc-binding dithiol moiety and an appendage that specifically binds to a pocket of ADAM17. We show that SN-4 inhibits the ability of ADAM17 to cleave tumor necrosis factor α (TNF-α) in vitro. This activity was reduced by the addition of zinc, indicating the importance of the zinc chelating dithiol moiety. Inhibition of TNF-α cleavage by SN-4 in cells was also observed, and with an IC50 of 3.22 µM, SN-4 showed slightly higher activity than the well-studied ADAM17 inhibitor marimastat. Furthermore, SN-4 was shown to inhibit cleavage of CD44 by ADAM17, but not by ADAM10, and to suppress cell invasion. Molecular docking showed good fitting of the specificity pocket-binding group and one SH of SN-4 and hinted at possible means of structural optimization. This study provides clues for the development of potent and selective ADAM17 inhibitors.

著者

Sugawara Y. Wright O. B. Matsuda O. Takigahira M. Tanaka Y. Tamura S. Gusev2 V. E.

出版者

American Physical Society

雑誌

PHYSICAL REVIEW LETTERS (ISSN:00319007)

巻号頁・発行日

vol.88, no.18, pp.185504, 2002-04-18

被引用文献数

106

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We present a new method for imaging surface phonon focusing and dispersion at frequencies up to 1 GHz that makes use of ultrafast optical excitation and detection. Animations of coherent surface phonon wave packets emanating from a point source on isotropic and anisotropic solids are obtained with micron lateral resolution. We resolve rounded-square shaped wave fronts on the (100) plane of LiF and discover isolated pockets of pseudosurface wave propagation with exceptionally high group velocity in the (001) plane of TeO2. Surface phonon refraction and concentration in a minute gold pyramid is also revealed.

著者

Michael W. Bolt Joseph T. Brady Lawrence O. Whiteley K. Nasir Khan

出版者

The Japanese Society of Toxicology

雑誌

The Journal of Toxicological Sciences (ISSN:03881350)

巻号頁・発行日

vol.46, no.2, pp.57-68, 2021 (Released:2021-02-02)

参考文献数

57

被引用文献数

1 22

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The number of gene therapies in development continues to increase, as they represent a novel method to treat, and potentially cure, many diseases. Gene therapies can be conducted with an in vivo or ex vivo approach, to cause gene augmentation, gene suppression, or genomic editing. Adeno-associated viruses are commonly used to deliver gene therapies, but their use is associated with several manufacturing, nonclinical and clinical challenges. As these challenges emerge, regulatory agency expectations continue to evolve. Following administration of rAAV-based gene therapies, nonclinical toxicities may occur, which includes immunogenicity, hepatotoxicity, neurotoxicity, and the potential risks for insertional mutagenesis and subsequent tumorgenicity. The mechanism for these findings and translation into the clinical setting are unclear at this time but have influenced the nonclinical studies that regulatory agencies are increasingly requesting to support clinical trials and marketing authorizations. These evolving regulatory expectations and toxicities, as well as future nonclinical considerations, are discussed herein.

著者

Schlitzer Reiner Anderson Robert F. Dodas Elena Masferrer Lohan Maeve Geibert Walter Tagliabue Alessandro Bowie Andrew Jeandel Catherine Maldonado Maria T. Landing William M. Cockwell Donna Abadie Cyril Abouchami Wafa Achterberg Eric P. Agather Alison Aguliar-Islas Ana van Aken Hendrik M. Andersen Morten Archer Corey Auro Maureen de Baar Hein J. Baars Oliver Baker Alex R. Bakker Karel Basak Chandranath Baskaran Mark Bates Nicholas R. Bauch Dorothea van Beek Pieter Behrens Melanie K. Black Erin Bluhm Katrin Bopp Laurent Bouman Heather Bowman Katlin Bown Johann Boyd Philip Boye Marie Boyle Edward A. Branellec Pierre Bridgestock Luke Brissebrat Guillaume Browning Thomas Bruland Kenneth W. Brumsack Hans-Jürgen Brzezinski Mark Buck Clifton S. Buck Kristen N. Buesseler Ken Bull Abby Butler Edward Cai Pinghe Mor Patricia Cámara Cardinal Damien Carlson Craig Carrasco Gonzalo Casacuberta Núria Casciotti Karen L. Castrillejo Maxi Chamizo Elena Chance Rosie Charette Matthew A. Chaves Joaquin E. Cheng Hai Chever Fanny Christl Marcus Church Thomas M. Closset Ivia Colman Albert Conway Tim M. Cossa Daniel Croot Peter Cullen Jay T. Cutter Gregory A. Daniels Chris Dehairs Frank Deng Feifei Dieu Huong Thi Duggan Brian Dulaquais Gabriel Dumousseaud Cynthia Echegoyen-Sanz Yolanda Edwards R. Lawrence Ellwood Michael Fahrbach Eberhard Fitzsimmons Jessica N. Russell Flegal A. Fleisher Martin Q. van de Flierdt Tina Frank Martin Friedrich Jana Fripiat Francois Fröllje Henning Galer Stephen J.G. Gamo Toshitaka Ganeshram Raja S. Garcia-Orellana Jordi Garcia-Solsona Ester Gault-Ringold Melanie George Ejin Gerringa Loes J.A. Gilbert Melissa Godoy Jose M. Goldstein Steven L. Gonzalez Santiago R. Grissom Karen Hammerschmidt Chad Hartman Alison Hassler Christel S. Hathorne Ed C. Hatta Mariko Hawco Nicholas Hayes Christopher T. Heimbürger Lars-Eric Helgoe Josh Heller Maija Henderson Gideon M. Henderson Paul B. van Heuven Steven Ho Peng Horner Tristan J. Hsieh Yu-Te Huang Kuo-Fang Humphreys Matthew P. Isshiki Kenji Jacquot Jeremy E. Janssen David J. Jenkins William J. John Seth Jones Elizabeth M. Jones Janice L. Kadko David C. Kayser Rick Kenna Timothy C. Khondoker Roulin Kim Taejin Kipp Lauren Klar Jessica K. Klunder Maarten Kretschmer Sven Kumamoto Yuichiro Laan Patrick Labatut Marie Lacan Francois Lam Phoebe J. Lambelet Myriam Lamborg Carl H. Le Moigne Frédéric A.C. Le Roy Emilie Lechtenfeld Oliver J. Lee Jong-Mi Lherminier Pascale Little Susan López-Lora Mercedes Lu Yanbin Masque Pere Mawji Edward Mcclain Charles R. Measures Christopher Mehic Sanjin Barraqueta Jan-Lukas Menzel van der Merwe Pier Middag Rob Mieruch Sebastian Milne Angela Minami Tomoharu Moffett James W. Moncoiffe Gwenaelle Moore Willard S. Morris Paul J. Morton Peter L. Nakaguchi Yuzuru Nakayama Noriko Niedermiller John Nishioka Jun Nishiuchi Akira Noble Abigail Obata Hajime Ober Sven Ohnemus Daniel C. van Ooijen Jan O'Sullivan Jeanette Owens Stephanie Pahnke Katharina Paul Maxence Pavia Frank Pena Leopoldo D. Peters Brian Planchon Frederic Planquette Helene Pradoux Catherine Puigcorbé Viena Quay Paul Queroue Fabien Radic Amandine Rauschenberg S. Rehkämper Mark Rember Robert Remenyi Tomas Resing Joseph A. Rickli Joerg Rigaud Sylvain Rijkenberg Micha J.A. Rintoul Stephen Robinson Laura F. Roca-Martí Montserrat Rodellas Valenti Roeske Tobias Rolison John M. Rosenberg Mark Roshan Saeed Rutgers van der Loeff Michiel M. Ryabenko Evgenia Saito Mak A. Salt Lesley A. Sanial Virginie Sarthou Geraldine Schallenberg Christina Schauer Ursula Scher Howie Schlosser Christian Schnetger Bernhard Scott Peter Sedwick Peter N. Semiletov Igor Shelley Rachel Sherrell Robert M. Shiller Alan M. Sigman Daniel M. Singh Sunil Kumar Slagter Hans A. Slater Emma Smethie William M. Snaith Helen Sohrin Yoshiki Sohst Bettina Sonke Jeroen E. Speich Sabrina Steinfeldt Reiner Stewart Gillian Stichel Torben Stirling Claudine H. Stutsman Johnny Swarr Gretchen J. Swift James H. Thomas Alexander Thorne Kay Till Claire P. Till Ralph Townsend Ashley T. Townsend Emily Tuerena Robyn Twining Benjamin S. Vance Derek Velazquez Sue Venchiarutti Celia Villa-Alfageme Maria Vivancos Sebastian M. Voelker Antje H.L. Wake Bronwyn Warner Mark J. Watson Ros van Weerlee Evaline Alexandra Weigand M. Weinstein Yishai Weiss Dominik Wisotzki Andreas Woodward E. Malcolm S. Wu Jingfeng Wu Yingzhe Wuttig Kathrin Wyatt Neil Xiang Yang Xie Ruifang C. Xue Zichen Yoshikawa Hisayuki Zhang Jing Zhang Pu Zhao Ye Zheng Linjie Zheng Xin-Yuan Zieringer Moritz Zimmer Louise A. Ziveri Patrizia Zunino Patricia Zurbrick Cheryl

出版者

Elsevier BV

雑誌

Chemical Geology (ISSN:00092541)

巻号頁・発行日

vol.493, pp.210-223, 2018-08-20

被引用文献数

256

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The GEOTRACES Intermediate Data Product 2017 (IDP2017) is the second publicly available data product of the international GEOTRACES programme, and contains data measured and quality controlled before the end of 2016. The IDP2017 includes data from the Atlantic, Pacific, Arctic, Southern and Indian oceans, with about twice the data volume of the previous IDP2014. For the first time, the IDP2017 contains data for a large suite of biogeochemical parameters as well as aerosol and rain data characterising atmospheric trace element and isotope (TEI) sources. The TEI data in the IDP2017 are quality controlled by careful assessment of intercalibration results and multi-laboratory data comparisons at crossover stations. The IDP2017 consists of two parts: (1) a compilation of digital data for more than 450 TEIs as well as standard hydrographic parameters, and (2) the eGEOTRACES Electronic Atlas providing an on-line atlas that includes more than 590 section plots and 130 animated 3D scenes. The digital data are provided in several formats, including ASCII, Excel spreadsheet, netCDF, and Ocean Data View collection. Users can download the full data packages or make their own custom selections with a new on-line data extraction service. In addition to the actual data values, the IDP2017 also contains data quality flags and 1-σ data error values where available. Quality flags and error values are useful for data filtering and for statistical analysis. Metadata about data originators, analytical methods and original publications related to the data are linked in an easily accessible way. The eGEOTRACES Electronic Atlas is the visual representation of the IDP2017 as section plots and rotating 3D scenes. The basin-wide 3D scenes combine data from many cruises and provide quick overviews of large-scale tracer distributions. These 3D scenes provide geographical and bathymetric context that is crucial for the interpretation and assessment of tracer plumes near ocean margins or along ridges. The IDP2017 is the result of a truly international effort involving 326 researchers from 25 countries. This publication provides the critical reference for unpublished data, as well as for studies that make use of a large cross-section of data from the IDP2017. This article is part of a special issue entitled: Conway GEOTRACES - edited by Tim M. Conway, Tristan Horner, Yves Plancherel, and Aridane G. González.

著者

金田 栄祐 平尾 邦雄 足原 修 清水 幹夫 伊藤 富造 小田 稔 KANEDA E. HIRAO K. ASHIHARA O. SHIMIZU M. ITOH T. ODA M.

出版者

宇宙科学研究所

雑誌

宇宙科学研究所報告. 特集: ハレー彗星探査計画報告 (ISSN:02859920)

巻号頁・発行日

vol.19, pp.111-124, 1987-03

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The Ultraviolet Imager aboard Suisei, the first Japanese interplanetary spacecraft, successfully ended ist mission by continuously observing the Ly α intensities of comet Halley. The major findings obtained from these observations are as follows : 1) Strong short-term variation of the Ly α intensities of comet Halley; Such a rapid variation was quite unexpected in the knowledge of the past Ly α observations of comet. In relation to this variation, an accurate determination of the rotation period of this comet was attempted (52.9 hours). 2) The first measurement of the Ly α intensities from within a thick hydrogen cloud of comet. While the intensity distribution as a function of the distance from the nucleus approximately follows that predicted by the Haser model, it is also compatible with the jet repetition model. 3) Existence of a fine structure in the above photometry data, which possibly represents local enhancements of the atomic hydrogen density. 4) Brightness variation within one rotational period was pursued, producing an outburst occurrence diagram. The rapid variation of the Ly α intensities as observed suggests that in an outburst event there must be involved a small amount of hydrogen-containing molecules whose photodissociation lifetimes are quite short. 5) The observed images are brighter (overally twice) than those expected from the nominal model by IACC/WG.

著者

Paula Goes Caio S. Dutra Mário R. P. Lisboa Delane V. Gondim Renata Leitão Gerly A. C. Brito Rodrigo O. Rego

出版者

Nihon University School of Dentistry

雑誌

Journal of Oral Science (ISSN:13434934)

巻号頁・発行日

vol.58, no.4, pp.569-574, 2016 (Released:2016-12-23)

参考文献数

29

被引用文献数

15 43

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This pilot study evaluated the clinical efficacy of a mouthwash containing 1% Matricaria chamomilla L. (MTC) extract in reducing gingival inflammation and plaque formation in patients undergoing orthodontic treatment with fixed appliances. This randomized, double-blind, placebo-controlled study enrolled a total of 30 males and females (age, 10-40 years) with fixed orthodontic appliances and a minimum of 20 natural teeth. The participants were allocated to three groups (n = 10 each) and asked to rinse with 15 mL of a placebo, 0.12% chlorhexidine (CHX), or 1% MTC mouthwash, immediately after brushing for 1 min, in the morning and evening, for 15 days. Data (mean ± SD) on visible plaque index (VPI) and gingival bleeding index (GBI) were recorded on days 1 and 15. The placebo group exhibited increases in VPI and GBI (10.2% and 23.1%, respectively) from day 1 to day 15. As compared with placebo, VPI and GBI significantly decreased in the MTC group (−25.6% and −29.9%, respectively) and the CHX group (−39.9% and −32.0%, respectively). In summary, MTC reduced biofilm accumulation and gingival bleeding in patients with gingivitis, probably because of its antimicrobial and anti-inflammatory activities.(J Oral Sci 58, 569-574, 2016)

著者

Marika Hausen ... [et al.] translated by Desmond O'Rourke Michael Wynne-Ellis and the English Centre

出版者

MIT Press

巻号頁・発行日

1990

著者

CLARIS O.

雑誌

Arch pediatr

巻号頁・発行日

vol.3, pp.452-456, 1996

被引用文献数

1 6

著者

竹松 正樹 下 相慶 VOLKOV Y.N. 崔 ぴょん昊 羅 貞烈 金 くー 金 慶烈 蒲生 俊敬 磯田 豊 DANCHENKOV M GONCHAREKO I CREPON M. LI RongーFeng JI ZhougーZhe ZATSEPIN A.G MILLOT C. SU JiーLan 尹 宗煥 OSTROVSKII A 松野 健 柳 哲雄 山形 俊男 野崎 義行 大谷 清隆 小寺山 亘 今脇 資郎 増田 章 YUNG John-fung BYOG S-k. NA J.-y. KIM K.r. CHOI Byong-ho CREPON Michel 崔 秉昊 金 丘 オストロフスキー A.G YURASOV G.I. 金子 新 竹内 謙介 川建 和雄 JIーLAN Su FENG Li Rong FEI Ye Long YARICHIN V.G PONOMAREV V. RYABOV O.A.

出版者

九州大学

雑誌

国際学術研究

巻号頁・発行日

1993

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1.本研究の特色は、研究分担者間の研究連絡・交流を促進し、研究成果の統合を図るに止まらず、研究課題に対する理解を格段に深めるのに必要な新しいデータセットの取得を提案し、実行することにある。実際、前年度の本研究主催の国際研究集会(福岡市)に於いて策定された計画に従い、平成6年7月に、日・韓・露の3国の参加を得て、日本海全域に亘る国際共同観測を実施した。この夏季観測では、海底までの精密CTD、化学計測、ドリフタ-の放流、測流用係留系(3本)の設置・回収と並んで、分担者の開発した特殊な曳航体を用いてクロロフィル、O_2、CO_2及び表層流速の測定が試みられた。特筆すべきは、日本海北部のロシア経済水域内の3点において、11ヶ月に及び長期測流データを取得したことである。これは、日本海誕生以来、初めて、日本海盆の中・深層の流動特性を明らかにしたもので貴重である。なお、現在、3本の係留計(うち1本にはセディメントトラップ付)が海中にあって測流中である。更に、冬期の過冷却による深層水の生成過程を調べる目的で、平成7年3月1日から、ウラジオストック沖において、3国共同観測が実施された。これは、次年度以降に予定されている本格的な冬季観測の準備観測として位置づけられている。2.現地観測による新しいデータセット取得の努力と並んで、室内実験に関する共同研究も活発に実施された。即ち、昨年度の研究集会での打ち合わせに従って、日・韓・露でそれぞれ冷却沈降過程に関する実験を進めるとともに、平成6年10月にはロシア・シルショフ研究所からディカレフ氏(研究協力者)を日本に招き、また、研究代表者が韓国漢陽大学を訪問し研究途中成果の比較検討を行った。この共同研究においては、特に、自由表面を確保する冷却(駆動)方法が試みられ、従来の固体表面を持つ実験と著しい差異があることを見出した。しかし、こうした実験結果を現実の沈降現象と結びつけるには、冬季における集中的現地観測の成果を持たねばならない。3.昨年1月の研究集会での検討・打合わせに従って、日・韓・仏・中・(米)の研究者により、日本海及び東シナ海域に関する数値モデル研究が共同で進められた。その成果として、東韓暖流の挙動(特に離岸現象)を忠実に再現できる新しい日本海数値モデルを開発するとともに、黒潮を含む東シナ海域の季節変動のメカニズムを解明するための数値モデル研究がなされた。4.夏季観測の際に放流したドリフタ-(アルゴスブイ)の挙動は韓国・成均館大学及び海洋研究所で受信され、衛星の熱赤外画像と高度計データは九州大学で連続的に収集された。こうした表層に関する情報を有機的に結合し、検討するため、平成7年1月に成均館大学の崔教授が九州大学を訪問した。5.以上の共同研究活動の全成果を多面的に検討し統合するために、平成6年11月7〜8日の2日間に亘り、韓国ソウル大学に於いて開催された国際研究集会に参加した。この集会には、日本・韓国・ロシアから、一般参加も含めて、約70名の参加者があった。参加者の専門分野が、海洋物理、化学、海洋工学及び生態学と多岐にわたっていることは学際性を標ぼうする本研究の特色を象徴するものである。アメリカからも2名の特別参加があり、本研究が口火を切った日本海及び東シナ海に関する学際的・国際的研究に対するアメリカの並々ならぬ関心が表明された。なお、ここで公表・検討された成果は、逐次、学術誌において印刷公表される予定である。過去2年に亘る本研究成果の総括もなされたが、そこでは、本研究が取得した新しいデータセットは、問題に解答を与えるというよりも、むしろ、新たな問題を提起する性質のものであることが認識された。そのため、最終セッションでは、提起された問題を解明して行くための今後の方策(主に現地観測)が詳細に検討された。

著者

Rod O’Donnell

出版者

The Keynes Society Japan

雑誌

The Review of Keynesian Studies (ISSN:24356581)

巻号頁・発行日

vol.2, pp.46-77, 2020 (Released:2021-04-24)

参考文献数

26

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Keynes and Paley are two names rarely conjoined in the literature of either figure. Keynes, however, made significant references to Paley in four of his essays. Most commentators treat these as historical ephemera, but closer investigations reveal that they illuminate key aspects of his thought. These include whether Paley or Malthus was the first Cambridge economist, methodological issues concerning the nature of economic theorising and how best to disseminate economic thought, and relationships between scientific and religious explanations. They also serve the useful task of clarifying and correcting certain errors in Keynes’s essays due to exaggeration or inaccuracy. In these contexts, Keynes’s remarks on Paley repay investigation.

著者

Shuai SUN Shota YANO Momo O NAKANISHI Michiko HIROSE Kazuhiko NAKABAYASHI Kenichiro HATA Atsuo OGURA Satoshi TANAKA

出版者

The Society for Reproduction and Development

雑誌

Journal of Reproduction and Development (ISSN:09168818)

巻号頁・発行日

pp.2020-119, (Released:2021-03-20)

被引用文献数

4

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Mouse trophoblast stem cells (TSCs) can differentiate into trophoblast cells, which constitute the placenta. Under conventional culture conditions, in a medium supplemented with 20% fetal bovine serum (FBS), fibroblast growth factor 4 (FGF4), and heparin and in the presence of mouse embryonic fibroblast cells (MEFs) as feeder cells, TSCs maintain their undifferentiated, proliferative status. MEFs can be replaced by a 70% MEF-conditioned medium (MEF-CM) or by TGF-ß/activin A. To find out if KnockOutTM Serum Replacement (KSR) can replace FBS for TSC maintenance, we cultured mouse TSCs in KSR-based, FBS-free medium and investigated their proliferation capacity, stemness, and differentiation potential. The results indicated that fibronectin, vitronectin, or laminin coating was necessary for adhesion of TSCs under KSR-based conditions but not for their survival or proliferation. While the presence of FGF4, heparin, and activin A was not sufficient to support the proliferation of TSCs, the addition of a pan-retinoic acid receptor inverse agonist and a ROCK-inhibitor yielded a proliferation rate comparable to that obtained under the conventional FBS-based conditions. TSCs cultured under the KSR-based conditions had a gene expression and DNA methylation profile characteristic of TSCs and exhibited a differentiation potential. Moreover, under KSR-based conditions, we could obtain a suspension culture of TSCs using extracellular matrix (ECM) coating-free dishes. Thus, we have established here, KSR-based culture conditions for the maintenance of TSCs, which should be useful for future studies.

著者

NIER A. O.

雑誌

Phys. Rev.

巻号頁・発行日

vol.77, pp.789-793, 1950

被引用文献数

17 786

著者

Sara Isaacson Ashley O’Brien Jennifer D. Lazaro Arlen Ray Gerard Fluet

出版者

The Society of Physical Therapy Science

雑誌

Journal of Physical Therapy Science (ISSN:09155287)

巻号頁・発行日

vol.30, no.4, pp.636-641, 2018 (Released:2018-04-20)

参考文献数

40

被引用文献数

5 17

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[Purpose] The aim of this study was to test the hypothesis that Lee Silverman Voice Treatment-BIG decreases the negative impact of hypokinesia on dual task performance in persons with Parkinson’s disease. [Subjects and Methods] The records of 114 patients with Parkinson’s admitted to outpatient rehabilitation at a suburban hospital were reviewed. Demographics and data for 8 outcome measures were extracted for subjects that completed 14 of 16 sessions of BIG. 93 of these subjects had records of pre and post-test Timed Up and Go, Timed Up and Go Motor, and Timed Up and Go Cognitive scores. Average age was 68.4 years (SD=10.6) and average disease duration was 4.9 years (SD=5.3). [Results] Subjects demonstrated statistically significant improvements for Timed Up and Go (3.3 SD=4.5), Timed Up and Go Motor (4.4 SD=5.8) and Timed Up and Go Cognitive (4.7 SD=5.4). Concurrent motor and cognitive performance remained stable. Dual task cost decreased at a statistically significant level for Timed Up and Go Cognitive (7% SD=31%) but not Motor (4% SD=32%). [Conclusion] These findings suggest that cueing strategies associated with LSVT BIG become internalized and decrease the negative impact of hypokinesia on mobility and cognitive performance while performing two tasks simultaneously in persons with Parkinson’s.

著者

Robert J. O'Neill foreword by Captain Sir Basil Liddell Hart

出版者

Cassell

巻号頁・発行日

1966

著者

William F. O'Connor

出版者

亜細亜大学総合学術文化学会

雑誌

亜細亜大学学術文化紀要 = Journal of the Society for General Academic and Cultural Research (ISSN:13470248)

巻号頁・発行日

vol.35, pp.129-140, 2019

著者

S.O.L.ラーゲルレーヴ作 前田晁訳

出版者

本の友社

巻号頁・発行日

2006

著者

酒井 達也 A. Jones Mark Smirnoff Nicholas 岡田 清孝 O. Wasteneys Geoffrey van der Honing Hannie 西岡 美樹 上原 由紀子 高橋 美穂子 藤澤 紀子 佐治 健介 関 原明 篠崎 一雄

出版者

日本植物生理学会

雑誌

日本植物生理学会年会およびシンポジウム 講演要旨集

巻号頁・発行日

vol.2008, pp.716, 2008

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キネシンモータータンパク質は様々な生物において細胞の先端成長及び細胞の形の決定に関与していることが知られている。シロイヌナズナゲノム中には 61 遺伝子のキネシン関連遺伝子が存在することが明らかになっているが、その多くは機能が明らかになっていない。我々はアルマジロリピートドメインを持つ植物特異的キネシン関連タンパク質 ARK1 がシロイヌナズナ根毛の先端成長に関与することを明らかにした。ark1 突然変異体根毛は波状、時に枝分かれの表現型を示し、内側の微小管はより重合して量及び長さが促進されていた。すなわちARK1は根毛内部の微小管量を限定する働きを持ち、これが根毛の先端成長を制御することが示唆された。ARK1 はARK2 及び ARK3 の二つのパラログ含む遺伝子ファミリーをシロイヌナズナゲノム中で形成しており、ARK2 が根の表皮細胞の形態形成に関与することを明らかにした。さらにARK タンパク質と結合する因子として NEK6 タンパク質リン酸化酵素を同定した。nek6 突然変異もまた、シロイヌナズナ表皮細胞の形態形成に多面的な影響を与えており、NEK6 が ARK キネシンに関連した微小管機能を介して細胞の形態形成に関与することが示唆された。