著者
Hiroshi IWANAGA Hirohiko TSUZUKI Yoichiro KAMIYAMA Hiroshi UEDA
出版者
(社)日本分析化学会
雑誌
Analytical Sciences (ISSN:09106340)
巻号頁・発行日
vol.25, no.9, pp.1101-1106, 2009-09-10 (Released:2009-09-10)
参考文献数
13
被引用文献数
1 2 3

With a specific and strong molecular-recognition capability cultivated in humoral acquired immunity, an antibody has been extensively utilized in various applications, such as diagnostics and therapy. However, so far most of its uses have been limited to be in the liquid phase. In view of its potential uses, such as a gas-phase biosensor or a high-performance air filter, we have tried to verify a previously undescribed binding reaction between protein antigens and corresponding antibodies immobilized on a solid surface by using fluorescence resonance energy transfer between the two. Our data showed that the antibody on a solid surface specifically reacted with a protein antigen supplied from the gas phase under the normal ambient condition. Also discovered was that the reaction occurred even faster than that in the liquid phase under several assay conditions.

言及状況

外部データベース (DOI)

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(続き)富士フイルムはダチョウ抗体固定化フィルターを搭載した空気清浄機の開発https://t.co/25Jsiimib3 の際フィルター=固体表面に固定化された抗体と空気中のウイルス粒子=抗原の抗原抗体反応を実証するためFRET(蛍光共鳴エネルギー移動)を利用する測定法https://t.co/X0ceeWNN2X まで開発
(富士フィルムは固体表面に固定化した抗体と空気中の抗原をそれぞれ蛍光色素で標識し、相互作用を検出する方法を論文http://t.co/T5V0jwPavg で報告。ただしこの論文で使用したのは抗原、抗体とも市販品で、ダチョウ抗体とインフルエンザウイルス抗原を使ったわけではない)

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