著者
Erwin Suwendi Hitoshi Iwaya Jae-Sung Lee Hiroshi Hara Satoshi Ishizuka
出版者
Biomedical Research Press
雑誌
Biomedical Research (ISSN:03886107)
巻号頁・発行日
vol.33, no.6, pp.329-336, 2012 (Released:2012-12-27)
参考文献数
28
被引用文献数
19 19

Dextran sulfate sodium (DSS)-induced colitis is an experimental model of ulcerative colitis, although the precise mechanism has not yet been elucidated. We investigate whether Zn deficiency affects the pathogenesis of colitis induced by DSS with a focus on immune responses. Male WKAH/Hkm Slc rats were fed either a Zn-adequate (ZA, 30 mg Zn/kg diet) as a control or Zndeficient (ZD, 5 mg Zn/kg diet) diet for 21 days and then treated with 2% DSS via deionized drinking water for 7 days. The disease activity index (DAI) was recorded daily throughout DSS treatment. Serum Zn concentrations were significantly lowered in rats fed the ZD diet than those fed the ZA diet at day 7 and 14. Surprisingly, DSS treatment considerably reduced the serum Zn in both groups. The rats fed the ZD diet showed exacerbated colitis based on clinical outcomes, including weight loss, increased DAI, and shortened colon length. An in vitro study corroborated these results, showing that a large amount of TNFα was induced by rat mesenteric leukocytes in response to lipopolysaccharide in ZD medium, but not in ZA medium. These results indicate that a modulation of TNFα production due to Zn deficiency influences disease activity in DSS-induced colitis. In addition, more attention should be given to Zn for prevention of colitis.

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亜鉛欠乏は実験的マウス結腸炎におけるサイトカイン産生の異常調整を誘発する 亜鉛欠乏食マウスはリポ多糖に応答してマウス腸間膜白血球によって大量のTNFαが誘導された。亜鉛欠乏によるTNFα産生の調節がDSS誘発性大腸炎の疾患活動性に影響を及ぼすことを示している。https://t.co/I18p4J8UKA

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