著者
Saori TAKAHASHI Taku YOSHIYA Kumiko YOSHIZAWA-KUMAGAYE Toshihiro SUGIYAMA
出版者
Biomedical Research Press
雑誌
Biomedical Research (ISSN:03886107)
巻号頁・発行日
vol.36, no.3, pp.219-224, 2015-06-01 (Released:2015-06-24)
参考文献数
23
被引用文献数
2 14

Angiotensin-converting enzyme 2 (ACE2) is a carboxypeptidase which is highly homologous to angiotensin-converting enzyme (ACE). ACE2 produces vasodilator peptides angiotensin 1-7 from angiotensin II. In the present study, we synthesized various internally quenched fluorogenic (IQF) substrates (fluorophore-Xaa-Pro-quencher) based on the cleavage site of angiotensin II introducing N-terminal fluorophore N-methylanthranilic acid (Nma) and C-terminal quencher Nε-2,4- dinitrophenyl-lysine [Lys(Dnp)]. The synthesized mixed substrates “Nma-Xaa-Pro-Lys(Dnp)” were hydrolyzed by recombinant human (rh) ACE2. The amount of each product was determined by liquid chromatography mass spectrometry (LC-MS) with fluorescence detection and it was found that Nma-His-Pro-Lys(Dnp) is the most suitable substrate for rhACE2. The Km, kcat, and kcat/Km values of Nma-His-Pro-Lys(Dnp) on rhACE2 were determined to be 23.3 μM, 167 s−1, and 7.17 μM−1 s−1, respectively. Using the rhACE2 and the newly developed IQF substrate, we found rhACE2 inhibitory activity in soybean and isolated the active compound soybean ACE2 inhibitor (ACE2iSB). The physicochemical data on the isolated ACE2iSB were identical to those of nicotianamine. ACE2iSB strongly inhibited rhACE2 activity with an IC50 value of 84 nM. This is the first demonstration of an ACE2 inhibitor from foodstuffs.

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ACE2阻害活性があることがわかったニコチアナミン(nicotianamine)。秋田県総合食品研究センターからの報告。別の埼玉からの報告では、大豆・インゲン・エンドウ豆などに多く含まれる。日本食では自然にAT1-7優位になるのかも。 https://t.co/ecgdUf58Sn https://t.co/scG5zHJHiF https://t.co/ohJgTfTkiQ
@ARanganathan72 @Sandeep_1966 Nicotianamine is a novel angiotensin-converting enzyme 2 inhibitor in soybean . Do Indians need to increase Soybean intake ? #COVID-19 in India https://t.co/nO0x6tT96o
Incredible. In 2015 Japanese scientists found a molecule from Soybean potently inhibiting human ACE2 that acts as a receptor for SARSCoV2. The IC50 is mind-numbing 84 nM. The molecule is Nicotianamine. How did we miss this? TRY IT, PLEASE, @Sandeep_1966 https://t.co/L9aYGpa18B https://t.co/d4JQcG4wGj
味噌汁は感染防御に効くと思うよw >https://t.co/8AIcACNhgm とか書くとまた怒られるんだろうかw デマだデマだってw まずは、読んで判断してほしいですよね。
日本人には、心のふるさとw「味噌汁」があるではないか! ACE2 inhibitorとしての活性を持つ味噌汁を飲んでくれww(デマではない) → J-STAGE Articles - <b>Nicotianamine is a novel angiotensin-converting enzyme 2 inhibitor in soy</b><b>bean </b> https://t.co/OJIxtOM44F

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