著者
前島 一博 城地 保昌 西野 吉則 高田 英昭 鎌田 福美 日原 さえら
出版者
一般社団法人 日本生物物理学会
雑誌
生物物理 (ISSN:05824052)
巻号頁・発行日
vol.53, no.1, pp.004-010, 2013 (Released:2013-01-29)
参考文献数
29

How is 2-m-long genomic DNA organized into a mitotic chromosome or nucleus? The nucleosome fiber has long been assumed to be folded into a 30-nm chromatin fiber, and further helically folded larger fiber. However, when we observed frozen hydrated human mitotic cells using cryoelectron microscopy (cryo-EM), no higher-order structures including 30-nm chromatin fibers were found. To further investigate the bulk structure of mitotic chromosomes, we performed small-angle X-ray scattering (SAXS) at SPring-8. No structural feature larger than 11 nm was detected, even on a chromosome-diameter scale (~1 μm). We also found a similar scattering pattern in interphase nuclei of HeLa cells in the range up to ~275 nm. Our findings suggest a common structural feature in interphase and mitotic chromatins: compact and irregular folding of nucleosome fibers occurs without a 30-nm chromatin structure.

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日本語の総説記事あった。 生物物理Vol.53 (2013) No.1 pp.004-010

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Jacques Dubochet https://t.co/FoVhHwjuAn https://t.co/ELWfgjERaZ https://t.co/GKfHqfgDuh
日本語の総説記事あった。 生物物理Vol.53 (2013) No.1 pp.004-010 / “ヒトゲノムDNAの不規則で柔軟な収納原理” http://t.co/V2fN2GH9mb

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