著者
Keisuke Fujita Mitsuhiro Iwaki
出版者
日本生物物理学会
雑誌
BIOPHYSICS (ISSN:13492942)
巻号頁・発行日
vol.10, pp.69-75, 2014 (Released:2014-11-01)
参考文献数
36

Myosin V is a vesicle transporter that unidirectionally walks along cytoskeletal actin filaments by converting the chemical energy of ATP into mechanical work. Recently, it was found that myosin V force generation is a composition of two processes: a lever-arm swing, which involves a conformational change in the myosin molecule, and a Brownian search-and-catch, which involves a diffusive “search” by the motor domain that is followed by an asymmetric “catch” in the forward actin target such that Brownian motion is rectified. Here we developed a system that combines optical tweezers with DNA nano-material to show that the Brownian search-and-catch mechanism is the energetically dominant process at near stall force, providing 13 kBT of work compared to just 3 kBT by the lever-arm swing. Our result significantly reconsiders the lever-arm swinging model, which assumes the swing dominantly produces work (>10 kBT), and sheds light on the Brownian search-and-catch as a driving process.

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【学会ニュース更新】欧文誌[BIOPHYSICS]に論文が新規掲載されました。(ブラウン型ナノマシンの作動原理 ―揺らぎの制御とエネルギー変換― ) https://t.co/ohkQWwLbnX

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