著者
Daiji KIYOZUMI Shunsuke YAGUCHI Junko YAGUCHI Atsuko YAMAZAKI Kiyotoshi SEKIGUCHI
出版者
Japanese Association for Laboratory Animal Science
雑誌
Experimental Animals (ISSN:13411357)
巻号頁・発行日
pp.21-0001, (Released:2021-04-06)

Sea urchin embryos have been one of model organisms to investigate cellular behaviors because of their simple cell composition and transparent body. They also give us an opportunity to investigate molecular functions of human proteins of interest that are conserved in sea urchin. Here we report that human disease-associated extracellular matrix orthologues ECM3 and QBRICK are necessary for mesenchymal cell migration during sea urchin embryogenesis. Immunofluorescence has visualized the colocalization of QBRICK and ECM3 on both apical and basal surface of ectoderm. On the basal surface, QBRICK and ECM3 constitute together a mesh-like fibrillar structure along the blastocoel wall. When the expression of ECM3 was knocked down by antisense-morpholino oligonucleotides, the ECM3-QBRICK fibrillar structure completely disappeared. When QBRICK was knocked down, the ECM3 was still present, but the basally localized fibers became fragmented. The ingression and migration of primary mesenchymal cells were not critically affected, but their migration at later stages was severely affected in both knock-down embryos. As a consequence of impaired primary mesenchymal cell migration, improper spicule formation was observed. These results indicate that ECM3 and QBRICK are components of extracellular matrix, which play important role in primary mesenchymal cell migration, and that sea urchin is a useful experimental animal model to investigate human disease-associated extracellular matrix proteins.

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Human disease-associated extracellular matrix orthologs ECM3 and QBRICK regulate primary mesenchymal cell migration in sea urchin embryos. https://t.co/yuDHYJvPdD #Morpholino
偶然同じ日に別の論文がExperimental Animalsにて掲載されました。ウニ胚ECMを構成するECM3とQBRICKが間充織細胞移動に関与することを示しました。阪大の浄住さんに透明なウニは間充織細胞移動の解析に最適かもとお誘いいただいて哺乳類ECM分子の機能をウニで調べたものです https://t.co/9drWuZt9MF

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