著者

山下 恵太郎

出版者

日本結晶学会

雑誌

日本結晶学会誌 (ISSN:03694585)

巻号頁・発行日

vol.64, no.2, pp.118-124, 2022-05-31 (Released:2022-06-01)

参考文献数

15

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Atomic model refinement and map calculation methods for cryo-EM single particle analysis(SPA)are explained in relation to X-ray crystallography. To make the best use of high-resolution reconstructions from SPA, it is important to calculate interpretable density maps to help build atomic models, and to use all experimental information to refine models. We developed a new program Servalcat to facilitate the structure refinement in reciprocal space. Servalcat performs structure refinement using REFMAC5, followed by calculation of weighted and sharpened Fo and Fo-Fc maps, which are useful in manual model rebuilding.