著者
Yutaka Tahara Katsuya Obara
出版者
Japan Poultry Science Association
雑誌
The Journal of Poultry Science (ISSN:13467395)
巻号頁・発行日
vol.51, no.3, pp.307-312, 2014-07-25 (Released:2014-07-25)

The development of shell-less culture methods for bird embryos with high hatchability would be useful for the efficient generation of transgenic chickens, embryo manipulations, tissue engineering, and basic studies in regenerative medicine. To date, studies of culture methods for bird embryos include the whole embryo culture using narrow windowed eggshells, surrogate eggshells, and an artificial vessel using a gas-permeable membrane. However, there are no reports achieving high hatchability of >50% using completely artificial vessels. To establish a simple method for culturing chick embryos with high hatchability, we examined various culture conditions, including methods for calcium supplementation and oxygen aeration. In the embryo cultures where the embryos were transferred to the culture vessel after 55-56 h incubation, more than 90% of embryos survived until day 17 when a polymethylpentene film was used as a culture vessel with calcium lactate and distilled water supplementations. The aeration of pure oxygen to the surviving embryos from day 17 yielded a hatchability of 57.1% (8 out of 14). Thus, we successfully achieved a high hatchability with this method in chicken embryo culture using an artificial vessel.

言及状況

外部データベース (DOI)

Google+ (1 users, 1 posts)

Hatching chicks without egg shells... ... seeing through the mysteries of life. Paper published in the Japan Poultry Science Association: https://www.jstage.jst.go.jp/article/jpsa/51/3/51_0130043 ...

はてなブックマーク (9 users, 10 posts)

ひよこがピヨの奴。やっぱ酸素透過度か。
“A Novel Shell-less Culture System for Chick Embryos Using a Plastic Film as Culture Vessels”

Twitter (176 users, 180 posts, 104 favorites)

知らなかった.2014年の論文.2013年には実現していた模様. "ニワトリの卵を殻なしで孵化させる方法" https://t.co/baAOPbzmDZ
El paper de los pollos sin cáscara está aquí https://t.co/MbOnou5ems https://t.co/EYkbz5sFZb
@ceticismo alias, nem esse, porque fazem suplementação de cálcio. https://t.co/qrGY28c0G6
The study on how chick embryos are hatched out of their shells https://t.co/QOl9whSr8a. Check out the clip here: https://t.co/aELNaQrblO
All I want for Christmas ... is an incubator so I can try this out!
Para aqueles q se interessaram pelo experimento sobre reprodução de aves em papel filme.... ^^ https://t.co/C08iecS9bW
Si alguien está interesado en el proyecto de encubar pollitos sin cascarón, acá está el #Paper https://t.co/UJhvjw64uM #educacion #Vida
論文が見つかった。難しそう。写真や図版が興味深い A Novel Shell-less Culture System for Chick Embryos Using a Plastic Film as Culture Vessels https://t.co/CpPzkCJeY8
@CK_Ariaze https://t.co/Q13BvUd0Zd
割れた卵を孵化させる論文これか https://t.co/Q13BvUd0Zd
#Science https://t.co/BcFL7jWl5Q A Novel Shell-less Culture System for Chick Embryos Using a Plastic Film as Culture Vessels
https://t.co/HXQjadWCtI A Novel Shell-less Culture System for Chick Embryos Using a Plastic Film as Culture Vessels #science
https://t.co/LHF5bsIhjr A Novel Shell-less Culture System for Chick Embryos Using a Plastic Film as Culture Vessel… https://t.co/yjMzl0jGU8
https://t.co/H3qvHIsy5T A Novel Shell-less Culture System for Chick Embryos Using a Plastic Film as Culture Vessels #science
A Novel Shell-less Culture System for Chick Embryos Using a Plastic Film as Culture Vessels #science https://t.co/5r5cEAulOe
que interesante esto, parece imposible pero lo hicieron https://t.co/Eocb7qh8Je
A Novel Shell-less Culture System for Chick Embryos Using a Plastic Film as Culture Vessels https://t.co/dKGnqMb1Wi
“A Novel Shell-less Culture System for Chick Embryos Using a Plastic Film as Culture Vessels” https://t.co/jOZJmnuuym
Twitterの仕様だと思うのですが、教えてもらったURLが残念ながらワークしないので、短縮URLで表示します。 https://t.co/VerjtMuPJ3 https://t.co/bNJ2Dauu6K / jpsadoi:10.2141/ jpsa.0130043
ひよこがピヨの奴。やっぱ酸素透過度か。 / “[PDF]A Novel Shell-less Culture System for Chick Embryos Using a Plastic Film as Culture Ve…” https://t.co/at6kpnVDIp
卵の殻を割ってから孵化させたスーパー高校生に世界中が注目 https://t.co/9SbOEAPhmP この論文を書いた先生は、参加した高校生の名前をすべて論文に載せるべきではないか。 https://t.co/1uQUJzKKeW
https://t.co/fSofzeFCXq
一部海外で話題になってYouTubeでも議論呼んでた卵の殻のない状態からのひよこの孵化の実験! 研究成果がちゃんとまとめられてる! https://t.co/v8LAJ5TnhJ
これが元論文かな.ニワトリ胚の殻なし培養 : A Novel Shell-less Culture System for Chick Embryos Using a Plastic Film as Culture Vessels https://t.co/LBUqX3Mr4n
卵の殻を割った状態でも発生が進む奴の論文みっけた https://t.co/MGreow2vjn
Cool video of students hatching a chick outside its shell https://t.co/W8tl6pmvqi Here's the paper: https://t.co/65AHyhjeru HT @CatCVicente
Shell-less hatching! https://t.co/OMbtCRLDUh
https://t.co/hUAx3VqsDE https://t.co/txVCMQLYJo >世界初となる、鶏の殻無し卵の孵化に成功しました。その成果が平成24年度の科学作品展論文の部で認められ、知事賞を受賞しました。
Hatching a chick outside of an egg https://t.co/sgNEaASwLu https://t.co/LzIRyZYpaj #asechat #biology https://t.co/TO1QW5S0ed
@AwwwNawMate @veronicamcc95 they didnae invent it, but, original's here: https://t.co/c4z8C9ZzF6
殻なしで雛を孵す研究の論文 https://t.co/YItEmTavFy
chicken without an egg. https://t.co/QFwbxgyUf1
Shell-less egg https://t.co/gQbGtQuk4j
This is some Jurassic Park level science! https://t.co/2pffBSznws
"A Novel Shell-less Culture System for Chick Embryos Using a Plastic Film as Culture Vessels -JPSA https://t.co/3rKQfbenK7
フェイスブックで静岡県の生物部の高校生たちが世界初の殻なしで有精卵をラップを代用して孵化に成功させたって注目されてて気になったから文書探してみたらあったので気になる方はぜひ!https://t.co/pXlXR9oLV1 英語版も https://t.co/niZnDP65x6
A Novel Shell-less Culture System for Chick Embryos Using a Plastic Film as Culture Vessels (link is to peer-revie… https://t.co/Gz0vGy7pxG
Here's the article of the shell-less chick hatching thing https://t.co/LXeNVsmrKM
A chicken born in a shell-less system. Is this the first step to the artificial womb? https://t.co/c3zOXwWSKe
Apparently you can break open an egg and still have a chick hatch without the shell! Very cool #science https://t.co/EXzl0oXVfl
日本高中實驗 沒有蛋殼的雞蛋也能孵出小雞 https://t.co/z33Yl3hAyC 來自 @YouTube 論文 https://t.co/fEgOGvU2Kn
Ini keren, hasil lab anak2 SMA masuk jurnal: https://t.co/B6pEKQ6Ac7 https://t.co/aJLlWOAD5w https://t.co/OJv0V6wqWq
番組見ると高校生が何かやったかのように見えるが、実際にはそうではない https://t.co/RgdOZ5oGNv
Shell-less Culture System for Chick Embryos Using a Plastic Film as Culture Vessels https://t.co/SyfvfgMiMy https://t.co/pVTSfzapol
rt> there's an actual paper about it https://t.co/RTQt169ZLT
Wombs next?...How to hatch chicks outside the shell https://t.co/0tYD8B7efu https://t.co/lXkCIaRobE
btol rupanya yang shell-less telur ayam tu. https://t.co/VrDF7KrCFD
https://t.co/2ynSTQU8gI 何でこんな人が偏差値44の’高校で働いてるのか不明w
発表論文「The Journal of Poultry Science Vol. 51 (2014) No. 3 p. 307-312」 @wp1t1 A Novel Shell-less Culture System・・・ https://t.co/WGV9pBycxu

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