著者
Yutaka Tahara Katsuya Obara
出版者
日本家禽学会
雑誌
The Journal of Poultry Science (ISSN:13467395)
巻号頁・発行日
vol.51, no.3, pp.307-312, 2014-07-25 (Released:2014-07-25)
参考文献数
14
被引用文献数
19

The development of shell-less culture methods for bird embryos with high hatchability would be useful for the efficient generation of transgenic chickens, embryo manipulations, tissue engineering, and basic studies in regenerative medicine. To date, studies of culture methods for bird embryos include the whole embryo culture using narrow windowed eggshells, surrogate eggshells, and an artificial vessel using a gas-permeable membrane. However, there are no reports achieving high hatchability of >50% using completely artificial vessels. To establish a simple method for culturing chick embryos with high hatchability, we examined various culture conditions, including methods for calcium supplementation and oxygen aeration. In the embryo cultures where the embryos were transferred to the culture vessel after 55-56 h incubation, more than 90% of embryos survived until day 17 when a polymethylpentene film was used as a culture vessel with calcium lactate and distilled water supplementations. The aeration of pure oxygen to the surviving embryos from day 17 yielded a hatchability of 57.1% (8 out of 14). Thus, we successfully achieved a high hatchability with this method in chicken embryo culture using an artificial vessel.

言及状況

外部データベース (DOI)

Google+ (1 users, 1 posts)

Hatching chicks without egg shells... ... seeing through the mysteries of life. Paper published in the Japan Poultry Science Association: https://www.jstage.jst.go.jp/article/jpsa/51/3/51_0130043 ...

はてなブックマーク (9 users, 10 posts)

ひよこがピヨの奴。やっぱ酸素透過度か。
“A Novel Shell-less Culture System for Chick Embryos Using a Plastic Film as Culture Vessels”

Twitter (534 users, 545 posts, 373 favorites)

플라스틱 컵과 랩을 이용하여 노른자를 부화하는 영상 : https://t.co/c4iYgPiXlx 해당 논문 : https://t.co/O65FKoAIX0 관심있으면 읽어봐 지금 이걸로 보고서 쓰는 중
@Joanillo66 @Ttuttuken https://t.co/K8evpWEcyd
W tym imponującym filmie z kliniki weterynaryjnej Mihama w Fukui w Japonii obserwujemy rozwój zarodka pisklęcia metodą wykluwania bez skorupy. Ta demonstracja jest praktykowana głównie na podstawie tego artykułu:https://t.co/ff9rRsAe7d [cały film:https://t.co/l69QwfeW11 ] https://t.co/kaHZC0PLJh
RT) https://t.co/xJYoRkfGMu 껍질없는 용기에 수정란을 병아리까지 배양 관찰
فيديو يظهر كيفية تطور جنين كتكوت
In this impressive video by the Mihama Veterinary Clinic in Fukui, Japan, we observe the development of a chick embryo by shell-less hatching method. This demonstration is practiced mainly based on this paper: https://t.co/PKLYDxiKz9 [full video: https://t.co/zkDd8pysa5] https://t.co/36YU0jNCmR
@kalen59 https://t.co/Ap6us8aVHU
@rafaelsolana2 Aquí el enlace al artículo. https://t.co/jrahlUBW5f
Aquí el enlace a un video donde unos estudiantes recrean el procedimiento descrito en el artículo científico cuyo enlace también cito, que describe la manera en que pueden incubarse embriones de aves fuera del cascarón. https://t.co/bzJ4MUtNdd https://t.co/jrahlUBW5f https://t.co/sonU74w7Df https://t.co/14Q3Bkhzpd
@OlegDemych1 @planetpng It's a very interesting article. https://t.co/Hu9JDcOrST
>безумный >ученый >вскрыл яйцо и вырастил цыпленка Там вообще-то два профессора, Ютака Тахара и Кацуя Обара, это их эксперимент с их студентами в 2016 году. И как всегда это делалось для вирусологии и прочей медицине Ссылка на статью https://t.co/ZKSapOPAPy https://t.co/8pdn56Dkzd
Pues sí que lo es, y parece que hay un artículo en el que explica todo el proceso (no lo he leído así que a lo mejor no lo dice todo) y os lo voy a dejar aquí por si tenés curiosidad https://t.co/qoWkQ4KX09
فيديو عجيب من عيادة بيطرية في اليابان يوضح مراحل تطور جنين (صوص) دجاجة بطريقة التفقيس بدون قشرة https://t.co/cPvooaorxh https://t.co/63EtpJ9YbF
In this impressive video by the Mihama Veterinary Clinic in Fukui, Japan, we observe the development of a chick embryo by shell-less hatching method. This demonstration is practiced mainly based on this paper: https://t.co/PKLYDxAlXJ [full video: https://t.co/zkDd8pQ3yF] https://t.co/90a3webeg5
Very interesting. Based on: Yutaka Tahara & Katsuya Obara - A Novel Shell-less Culture System for Chick Embryos Using a Plastic Film as Culture Vessels https://t.co/mNh8vn6oS7 Paper discusses hatchability & considerations not shown in video guide. https://t.co/YjdzXVRiTf
This is certainly something I would love to try myself! Good idea for a high school project? Paper: Y. Tahara & K. Obara 2014, https://t.co/PW2v63sZgZ Video: https://t.co/En7dxEbVmv #Science #FunExperiments
这个实验发在2014年发在: https://t.co/M3p1qwkeTm 在此之前也还有很多, 最早可能是1971年的这篇: https://t.co/RulktwkPEl 这里还有一篇1993年的综述步骤写得也很清楚. #看起来很好玩啊 #没人接着玩光遗传学么 https://t.co/bcuBXqlFPT
Bookmark: 透明カップでの卵の孵し方 https://t.co/gjozuuWSgf 記事 https://t.co/zvJGPsmUkh ニュース動画 https://t.co/9CVQUu6InW
https://t.co/FEWAW3xWv9 https://t.co/LdmlqDC3F9
知らなかった.2014年の論文.2013年には実現していた模様. "ニワトリの卵を殻なしで孵化させる方法" https://t.co/baAOPbzmDZ
@ceticismo alias, nem esse, porque fazem suplementação de cálcio. https://t.co/qrGY28c0G6
The study on how chick embryos are hatched out of their shells https://t.co/QOl9whSr8a. Check out the clip here: https://t.co/aELNaQrblO
A Novel Shell-less Culture System for Chick Embryos Using a Plastic Film as Culture Vessels. https://t.co/N16ZsZrYqV
Shell-less Chick Embryo Culture System. https://t.co/N16ZsZrYqV
All I want for Christmas ... is an incubator so I can try this out!
これか / A Novel Shell-less Culture System for Chick Embryos Using a Plastic Film as Culture Vessels: https://t.co/rlUBkk4Neo
Para aqueles q se interessaram pelo experimento sobre reprodução de aves em papel filme.... ^^ https://t.co/C08iecS9bW
Si alguien está interesado en el proyecto de encubar pollitos sin cascarón, acá está el #Paper https://t.co/UJhvjw64uM #educacion #Vida
論文が見つかった。難しそう。写真や図版が興味深い A Novel Shell-less Culture System for Chick Embryos Using a Plastic Film as Culture Vessels https://t.co/CpPzkCJeY8
@CK_Ariaze https://t.co/Q13BvUd0Zd
割れた卵を孵化させる論文これか https://t.co/Q13BvUd0Zd
#Science https://t.co/BcFL7jWl5Q A Novel Shell-less Culture System for Chick Embryos Using a Plastic Film as Culture Vessels
https://t.co/HXQjadWCtI A Novel Shell-less Culture System for Chick Embryos Using a Plastic Film as Culture Vessels #science
https://t.co/LHF5bsIhjr A Novel Shell-less Culture System for Chick Embryos Using a Plastic Film as Culture Vessel… https://t.co/yjMzl0jGU8
@TsuboiA JStage 掲載論文です。 全文英語ですね。 https://t.co/dXy0qg8Pvg
https://t.co/H3qvHIsy5T A Novel Shell-less Culture System for Chick Embryos Using a Plastic Film as Culture Vessels #science
A Novel Shell-less Culture System for Chick Embryos Using a Plastic Film as Culture Vessels #science https://t.co/5r5cEAulOe
A Novel Shell-less Culture System for Chick Embryos Using a Plastic Film as Culture Vessels https://t.co/dKGnqMb1Wi
“A Novel Shell-less Culture System for Chick Embryos Using a Plastic Film as Culture Vessels” https://t.co/jOZJmnuuym
Twitterの仕様だと思うのですが、教えてもらったURLが残念ながらワークしないので、短縮URLで表示します。 https://t.co/VerjtMuPJ3 https://t.co/bNJ2Dauu6K / jpsadoi:10.2141/ jpsa.0130043
ひよこがピヨの奴。やっぱ酸素透過度か。 / “[PDF]A Novel Shell-less Culture System for Chick Embryos Using a Plastic Film as Culture Ve…” https://t.co/at6kpnVDIp
卵の殻を割ってから孵化させたスーパー高校生に世界中が注目 https://t.co/9SbOEAPhmP この論文を書いた先生は、参加した高校生の名前をすべて論文に載せるべきではないか。 https://t.co/1uQUJzKKeW
これが元論文かな.ニワトリ胚の殻なし培養 : A Novel Shell-less Culture System for Chick Embryos Using a Plastic Film as Culture Vessels https://t.co/LBUqX3Mr4n
卵の殻を割った状態でも発生が進む奴の論文みっけた https://t.co/MGreow2vjn
Cool video of students hatching a chick outside its shell https://t.co/W8tl6pmvqi Here's the paper: https://t.co/65AHyhjeru HT @CatCVicente
Shell-less hatching! https://t.co/OMbtCRLDUh
Hatching a chick outside of an egg https://t.co/sgNEaASwLu https://t.co/LzIRyZYpaj #asechat #biology https://t.co/TO1QW5S0ed
@AwwwNawMate @veronicamcc95 they didnae invent it, but, original's here: https://t.co/c4z8C9ZzF6
殻なしで雛を孵す研究の論文 https://t.co/YItEmTavFy
chicken without an egg. https://t.co/QFwbxgyUf1
Shell-less egg https://t.co/gQbGtQuk4j
This is some Jurassic Park level science! https://t.co/2pffBSznws
"A Novel Shell-less Culture System for Chick Embryos Using a Plastic Film as Culture Vessels -JPSA https://t.co/3rKQfbenK7
フェイスブックで静岡県の生物部の高校生たちが世界初の殻なしで有精卵をラップを代用して孵化に成功させたって注目されてて気になったから文書探してみたらあったので気になる方はぜひ!https://t.co/pXlXR9oLV1 英語版も https://t.co/niZnDP65x6
A Novel Shell-less Culture System for Chick Embryos Using a Plastic Film as Culture Vessels (link is to peer-revie… https://t.co/Gz0vGy7pxG
A chicken born in a shell-less system. Is this the first step to the artificial womb? https://t.co/c3zOXwWSKe
Apparently you can break open an egg and still have a chick hatch without the shell! Very cool #science https://t.co/EXzl0oXVfl
日本高中實驗 沒有蛋殼的雞蛋也能孵出小雞 https://t.co/z33Yl3hAyC 來自 @YouTube 論文 https://t.co/fEgOGvU2Kn
Ini keren, hasil lab anak2 SMA masuk jurnal: https://t.co/B6pEKQ6Ac7 https://t.co/aJLlWOAD5w https://t.co/OJv0V6wqWq
番組見ると高校生が何かやったかのように見えるが、実際にはそうではない https://t.co/RgdOZ5oGNv
Shell-less Culture System for Chick Embryos Using a Plastic Film as Culture Vessels https://t.co/SyfvfgMiMy https://t.co/pVTSfzapol
rt> there's an actual paper about it https://t.co/RTQt169ZLT
Wombs next?...How to hatch chicks outside the shell https://t.co/0tYD8B7efu https://t.co/lXkCIaRobE
btol rupanya yang shell-less telur ayam tu. https://t.co/VrDF7KrCFD

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