著者
Shinya AYABE Kenichi NAKASHIMA Atsushi YOSHIKI
出版者
THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT
雑誌
Journal of Reproduction and Development (ISSN:09168818)
巻号頁・発行日
pp.2018-128, (Released:2018-12-06)
被引用文献数
22

Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas-based genome editing technology has enabled manipulation of the embryonic genome. Unbiased whole genome sequencing comparing parents to progeny has revealed that the rate of Cas9-induced mutagenesis in mouse embryos is indistinguishable from the background rate of de novo mutation. However, establishing the best practice to confirm on-target alleles of interest remains a challenge. We believe that improvement in editing strategies and screening methods for founder mice will contribute to the generation of quality-controlled animals, thereby ensuring reproducibility of results in animal studies and advancing the 3Rs (replacement, reduction, and refinement).

言及状況

外部データベース (DOI)

Twitter (21 users, 21 posts, 16 favorites)

収集済み URL リスト