著者
Yu Nakajima Keiichi Kojima Yuichiro Kashiyama Satoko Doi Ryosuke Nakai Yuki Sudo Kazuhiro Kogure Susumu Yoshizawa
出版者
Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles
雑誌
Microbes and Environments (ISSN:13426311)
巻号頁・発行日
vol.35, no.4, pp.ME20085, 2020 (Released:2020-12-05)
参考文献数
36

Microbial rhodopsins, comprising a protein moiety (rhodopsin apoprotein) bound to the light-absorbing chromophore retinal, function as ion pumps, ion channels, or light sensors. However, recent genomic and metagenomic surveys showed that some rhodopsin-possessing prokaryotes lack the known genes for retinal biosynthesis. Since rhodopsin apoproteins cannot absorb light energy, rhodopsins produced by prokaryotic strains lacking genes for retinal biosynthesis are hypothesized to be non-functional in cells. In the present study, we investigated whether Aurantimicrobium minutum KNCT, which is widely distributed in terrestrial environments and lacks any previously identified retinal biosynthesis genes, possesses functional rhodopsin. We initially measured ion transport activity in cultured cells. A light-induced pH change in a cell suspension of rhodopsin-possessing bacteria was detected in the absence of exogenous retinal. Furthermore, spectroscopic analyses of the cell lysate and HPLC-MS/MS analyses revealed that this strain contained an endogenous retinal. These results confirmed that A. minutum KNCT possesses functional rhodopsin and, hence, produces retinal via an unknown biosynthetic pathway. These results suggest that rhodopsin-possessing prokaryotes lacking known retinal biosynthesis genes also have functional rhodopsins.

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外部データベース (DOI)

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とある微生物は既知のレチナール合成遺伝子を持たないのに、レチナールを合成してちゃんとロドプシンを機能させるらしい 未知のレチナール合成遺伝子があるかもしれないとな https://t.co/YTLxtR3Xsp
既知レチナール合成遺伝子を持たないバクテリアが作るロドプシンは働くのか?働かないのか? その一つの答えになる論文が出版されました。 Bacterium Lacking a Known Gene for Retinal Biosynthesis Constructs Functional Rhodopsins https://t.co/IXrx65GZhQ
Bacterium Lacking a Known Gene for Retinal Biosynthesis Constructs Functional Rhodopsins https://t.co/zntZESgCD3

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