著者

山本 俊夫 島津 智浩 遠藤 善宏 白石 廣行 影山 努

出版者

公益社団法人 日本水環境学会

雑誌

水環境学会誌 (ISSN:09168958)

巻号頁・発行日

vol.28, no.8, pp.515-521, 2005-08-10 (Released:2008-01-22)

参考文献数

25

被引用文献数

1 1

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The concentrations of norovirus (NV) genes in water samples from rivers A, B and C flowing into oyster farms in Miyagi Prefecture, Japan were determined from October 2002 to March 2004. Norovirus genes in 35 specimens collected over 17 months were measured using genogroup 1 (G1) and genogroup 2 (G2) specific real-time quantitative reverse transcription PCR techniques. The respective positive rates of NV genes in rivers A, B and C were 51% (18 of 35), 97% (34 of 35) and 26% (9 of 35). The respective concentrations of the NV G1 gene ranged from 85 - 5.3 × 103 copies, 80 - 9.6 × 104 copies and 85 - 1.7 × 103 copies in 100 ml of water samples from rivers A, B and C. The respective concentrations of the NV G2 gene ranged from 93 - 2.0 × 103 copies, 65 - 1.5 × 105 copies and 72 - 1.2 × 103 copies in 100 ml of the same water sample. These values were high during winter, and low but detectable during summer.