著者
Hirotaka TOMIYASU Yuko GOTO-KOSHINO Yasuhito FUJINO Koichi OHNO Hajime TSUJIMOTO
出版者
公益社団法人 日本獣医学会
雑誌
Journal of Veterinary Medical Science (ISSN:09167250)
巻号頁・発行日
pp.13-0337, (Released:2013-10-25)
被引用文献数
4 4

Treatments for canine lymphoma often fail because tumor cells acquire multidrug resistance (MDR). MDR can develop through several mechanisms, among which the overexpression of drug transporters in tumor cells is a well-studied mechanism. ATP-binding cassette sub-family G member 2 (ABCG2) belongs to the ABC-transporters, that are representative drug efflux pumps associated with MDR in human tumor cells. However, the regulation of ABCG2 gene expression in canine tumors is not well understood. The purpose of the present study was to reveal the regulatory mechanism of ABCG2 gene expression in 4 canine lymphoid tumor cell lines, GL-1, CLBL-1, UL-1 and Ema. Treatment with phorbol 12-myristate 13-acetate (PMA), the protein kinase C (PKC) activator, stimulated MAPK/ERK pathway in GL-1, UL-1 and Ema cells and JNK pathway in UL-1 and Ema cells. When GL-1 and UL-1 cells were treated with PMA and the MAPK/ERK kinase inhibitor U0126, ABCG2 gene expression levels were elevated above those in untreated cells. Similarly, ABCG2 gene expression increased above control levels in UL-1 and Ema cells treated with PMA and the JNK inhibitor SP600125. However, ABCG2 gene expression was unaffected by U0126 exposure in CLBL-1 cells, in which activation of MAPK/ERK pathway was observed in non-treated cells. These results suggested that MAPK/ERK and JNK pathways downregulate ABCG2 gene expression, which is upregulated by unidentified but possibly PKC-dependent pathways, in several types of canine lymphoid tumor cells.

言及状況

外部データベース (DOI)

Twitter (2 users, 3 posts, 0 favorites)

MAPK/ERK and JNK pathways downregulated ABCG2 gene expression in canine lymphoid tumor cell lines https://t.co/jxEu4ASZxe
Expression regulation of ABCG2 through mitogen-activated protein kinase pathways in canine lymphoid tumor cell lines https://t.co/jxEu4ASZxe
MAPK/ERK & JNK pathways downregulate ABCG2 gene expression in several types of canine #lymphoid_tumor cells http://t.co/ZQIf5HPytB

収集済み URL リスト