著者

Shinzo Tsuda Yuko Fukui Noriko Nakamura Yukihisa Katsumoto Keiko Yonekura-Sakakibara Masako Fukuchi-Mizutani Kazuyuki Ohira Yukiko Ueyama Hideo Ohkawa Timothy A. Holton Takaaki Kusumi Yoshikazu Tanaka

出版者

日本植物細胞分子生物学会

雑誌

Plant Biotechnology (ISSN:13424580)

巻号頁・発行日

vol.21, no.5, pp.377-386, 2004 (Released:2005-06-03)

参考文献数

34

被引用文献数

35 73

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Petunia flower colors are mainly due to flavonoids. The flower color of commercial varieties of Petunia hybrida was successfully modified by the suppression of endogenous flavonoid biosynthetic genes, the expression of a hetelorogous flavonoid biosynthetic gene, and the combination of both. Flower color changed from purple to almost white or from purple to red by the suppression of the endogenous gene expression, from red to orange by the down-regulation of the flavonoid 3′-hydroxylase gene and the expression of the rose dihydroflavonol 4-reductase gene, and from violet to pale violet by the expression of the flavonol synthase or flavone synthase gene. These results clearly indicate the usefulness of metabolic engineering of the flavonoid biosynthetic pathway to modify flower color. Only a few of the transgenic petunia exhibited phenotypic stability. For commercialisation, it is necessary to generate many independent transgenic lines, select elite lines with stable phenotypes and maintain them in tissue culture.