著者
Ryozo Imai Haruyasu Hamada Yuelin Liu Qianyan Linghu Yuya Kumagai Yozo Nagira Ryuji Miki Naoaki Taoka
出版者
Japanese Society for Plant Biotechnology
雑誌
Plant Biotechnology (ISSN:13424580)
巻号頁・発行日
vol.37, no.2, pp.171-176, 2020-06-25 (Released:2020-06-25)
参考文献数
27

Transformation is a key step in modern breeding technology that involves genome editing. The requirement for in vitro tissue culture and regeneration hampers application of this technology to commercially important varieties of many crop species. To overcome this problem, we developed a simple and reproducible in planta transformation method in wheat (Tritticum aestivum L.). Our in planta particle bombardment (iPB) method utilizes the shoot apical meristem (SAM) as a target tissue. The SAM contains a subepidermal cell layer termed L2, from which germ cells later develop during floral organogenesis. The iPB method can also be used for genome editing through transient CRISPR/Cas9 expression or direct delivery of the CRISPR/Cas9 ribonucleoprotein. In this review, we describe the iPB technology and provide an overview of its current and future applications in plant transformation and genome editing.

言及状況

外部データベース (DOI)

Twitter (7 users, 7 posts, 13 favorites)

A review article on our in planta technology is out. https://t.co/68LClsp7YJ https://t.co/8iHzWFbOE9

収集済み URL リスト