著者

大島 悦男 佐藤 秀幸 小場瀬 宏之 内村 達雄 桑原 隆 小林 智

出版者

The Pharmaceutical Society of Japan

雑誌

Chemical and Pharmaceutical Bulletin (ISSN:00092363)

巻号頁・発行日

vol.40, no.9, pp.2552-2554, 1992-09-25 (Released:2008-03-31)

参考文献数

8

被引用文献数

2 6

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(Z)-11-[3-(Dimethlamino)propylidene]-2-(methoxycarbonyl)methyl-6, 11-dihydrodibenz[b, e]oxepin-9-acrylic acid (5) was prepared for application to the radiommunoassay of KW-4679 (1, (Z)-11-[3-(dimethylamino)propylidene]-6, 11-dihydrodibenz[b, e]oxepin-2-acetic acid hydrochloride). The acrylic acid moiety in the 9-position of 5 was employed for coupling with an amino group of bovine serum albumin (BSA) to provide 17. Subsequently, the conjugate 17 was treated with aquenus NaOH to hydrolyze the terminal methoxycarbonyl group in the 2-position of the BSA conjugated 5. Antiserum raised against the antigenic BSA-conjugate 4 finally obtained was specific for 1.