著者

酒井 一夫 岡田 重文

出版者

日本放射線影響学会

雑誌

Journal of Radiation Research (ISSN:04493060)

巻号頁・発行日

vol.22, no.4, pp.415-424, 1981

被引用文献数

13 15

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An alkaline separation technique originally established by Ahnstrom is modified to detect small amount of DNA damage in X-irradiated mouse leukemic L5178Y cells. It is made quantitative by calibration with an alkaline sucrose gradient centrifugation. The present method would make it possible to study DNA damage and its repair within a dose range of X-rays where cell survival and mutation are usually investigated. It is also useful for detecting DNA damage caused by chemicals.