著者

望月 肇 賀田 恒夫

出版者

日本放射線影響学会

雑誌

Journal of Radiation Research (ISSN:04493060)

巻号頁・発行日

vol.23, no.3, pp.403-410, 1982-09

被引用文献数

4

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We formerly showed that human placenta contained strong antimutagenic factors working on radiation and chemically induced mutations in bacteria We are actually examining, in several aspects, if these factors might have certain physiological functions in mammals or not. In this communication, we report that our preparations made from the human placenta tissues greatly restore lethal injuries in X-ray-irradiated mice. Three placenta specimens of healthy women were obtained from the Shimada Hospital (Mishima City). Each tissue was first washed with aqueous KCl (0.15 M) solution, homogenized without any supplementation and centrifuged at 14,000 x g for 20 minutes. The supernantant was then treated with 20 μig/ml pronase at 370C for 20 minutes, followed by dialysis overnight in distilled water. The dialysate was then heated in boiling water for 30 minutes to denature proteic constituents and centrifuged at 3000 x g for 20 minutes. The cellular supernatant solution in a total volume of 10 ml was loaded first on a column (2×20 cm) of Amberlite IR-120B resin and the column was eluted with 500 ml of distilled water.