著者

Masaki Tadakatsu Endo Katsuhiko Kumagai Kanji

出版者

Zoological Society of Japan

雑誌

Zoological Science (ISSN:02890003)

巻号頁・発行日

vol.5, no.5, pp.p1051-1057, 1988-10

被引用文献数

1

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In the butterfly, Polygonia c-aureum L., a physiological mechanism underlying the photoperiodic control of seasonal-morph development involves a summer-morph-producing factor (SMPH). Amounts of SMPH present in the brain-extracts of Polygonia pupae as well as in those of the silkmoth. Bombyx mori, were evaluated by Polygonia pupal assay for SMPH. The factors responsible for SMPH-activity in the Polygonia and Bombyx brain-extracts were heat-stable (95℃ for 5min), but they were thought to be peptide hormones since the brain-extracts became SMPH-inactive by hydrolyzing with trypsin (24hr at 25℃). According to the Sephadex G-50 gel-filtration pattern of SMPH-activity, the ultimate molecular weight of SMPH was though to be 4500, which is almost the same size as the small prothoracicotropic hormone purified from the silkmoth, B. mori (4K-PTTH or bombixin: M. W. 4400). This finding is also supported by evidence in our present study that 2 (out of 4) Sephadex G-50 fractions judged as being SMPH-active showed 4K-PTTH-activity by Papilio pupal assay. However, the factor showing SMPH-activity was not thought to be identical with the one showing 4K-PTTH-activity in P. c-aureum since they were separated by reversed-phase HPLC.