著者
Uchida Eriko Mizuguchi Hiroyuki Ishii-Watabe Akiko HAYAKAWA Takao
出版者
公益社団法人日本薬学会
雑誌
Biological & pharmaceutical bulletin (ISSN:09186158)
巻号頁・発行日
vol.25, no.7, pp.891-897, 2002-07-01
被引用文献数
3 96

Non-viral gene transfer into a wide range of human cells was examined in order to clarify the factors that affect the efficiency and safety of non-viral vectors and to optimize the conditions so that high efficiency and low toxicity could be achieved. Six non-viral vectors (Lipofectin, LipofectAMINE PLUS, SuperFect, Effectene, DMRIE-C and DOTAP) were used to transfect a mammalian expression plasmid pCMVβ into 16 types of human primary cells and cultured cell lines. Transfection efficiency was quantified using a galactosidase assay. Cytotoxic effects were measured by lactate dehydrogenase (LDH) assay and WST-8 assay. In serum-free conditions, LipofectAMINE PLUS, Effectene and SuperFect, on average, transfected DNA more successfully than Lipofectin, DMRIE-C, and DOTAP, although the levels of gene expression with these vectors varied remarkably in different cells. The most effective vector also differed depending on the cell type. Serum was found to inhibit gene transfer and reduce the cytotoxicity of all of these vectors except Effectene. The efficiency and toxicity of the non-viral vectors used depended on the type of vector, the DNA/vector ratio, the type of cell, and the presence of serum. These results provided useful information for the optimization of transfer conditions of these non-viral vectors.

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何を用いてどのような方法でどのような遺伝子をどのような細胞に導入するかによって効率もまちまちですので、以下の文献などを参考に、遺伝子導入方法、導入細胞、など絞ってお調べになる必要があろうかと思います。 方法によって異なる細胞への導入効率が異なるという例: Mol Imaging Biol. 2010 Jan-Feb;12(1):15-24. doi: 10.1007/s11307-009- ...

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