著者

神津 公

出版者

公益社団法人日本薬学会

雑誌

衛生化学 (ISSN:0013273X)

巻号頁・発行日

vol.28, no.2, pp.111-113, 1982-02-28

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Caffeine and sodium benzoate were determined by high performance liquid chromatography with a UV detector by use of stainless column (4.0 mm×0.15 m) packed with Nucleosil _5C_<18>. Mobile phase was M/30 KH_2PO_4+M/30 Na_2HPO_4・2H_2O-methanol (70 : 30). The sample was dissolved in 20% aqueous methanol. Determination was carried out successfully by using antipyrine as an internal standard and by maintaining the temperature at 40℃. The recovery of sodium benzoate and caffeine from synthetic mixtures were 97.2±1.0% and 99.8±1.1%. This method was applied to three kinds of pharmaceutical preparations. The contents of sodium benzoate and caffeine were 48.4-50.1% and 49.0-49.6%.