著者

黒田 久寅 熊野 明美 岡本 幸子

出版者

公益社団法人日本薬学会

雑誌

衛生化学 (ISSN:0013273X)

巻号頁・発行日

vol.10, no.3, pp.165-168, 1964-09-30

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The present work was undertaken to clarify the physiological mechanism of germination of conidio spore of Cochliobolus miyabeanus. With the spore obtained by wet method, rate of germinaton and regression coefficient of germination curve decreased and the synchronism of spore lowered during storage. But, with the spore obtained by dry method, this phenomena could not be recognized even after 12 weeks'storage. Neither α-picolinic acid nor its analogues were found in the spore. This spore showed a great resistance to such respiration poison as potassium cyanide and sodium azide, therefore, under the condition completely inhibiting the oxygen-uptake (concentration of KCN or NaN_3 was 10^<-3>M/L) or without oxygen the formation of germ tube was observed. Thus, it was supposed that this spore had a metabolic pathway producing energy necessary for germination under an anaerobic condition. The respiration of spore rapidly increased instantly after the beginning of cultivation and the faculty to oxidize external substrate had a tendency to increase after the formation of germ tube.