- 著者
-
Namangala Boniface
Hachaambwa Lottie
Kajino Kiichi
Mweene Aaron S.
Hayashida Kyouko
Simuunza Martin
Simukoko Humphrey
Choongo Kennedy
Chansa Pamela
Lakhi Shabir
Moonga Ladslav
Chota Amos
Ndebe Joseph
Nsakashalo-Senkwe Mutale
Chizema Elizabeth
Kasonka Lackson
Sugimoto Chihiro
- 出版者
- BioMed Central
- 雑誌
- Parasites & Vectors (ISSN:17563305)
- 巻号頁・発行日
- vol.5, 2012-12-04
- 被引用文献数
-
1
35
Background: Loop-mediated isothermal amplification (LAMP) is a novel strategy which amplifies DNA with high sensitivity and rapidity under isothermal conditions. In the present study, the performance of the repetitive insertion mobile element (RIME)-LAMP and human serum resistance-associated gene (SRA)-LAMP assays were evaluated using clinical specimens obtained from four male patients from Luangwa and Zambezi valleys in Zambia and Zimbabwe, respectively. Findings: The cases reported in this preliminary communication were all first diagnosed by microscopy, through passive surveillance, and confirmed by both RIME-LAMP and SRA-LAMP. A good correlation between microscopy and LAMP was observed and contributed to staging and successful treatment of patient. RIME-LAMP and SRA-LAMP complimented each other well in all the cases. Conclusions: Both RIME-LAMP and SRA-LAMP were able to detect Trypanosoma brucei rhodesiense DNA in patient blood and CSF and hence confirmed HAT in the parasitaemic patients. Our study indicates that the LAMP technique is a potential tool for HAT diagnosis, staging and may be useful for making therapeutic decisions. However, no statistically significant conclusion may be drawn due to the limited sample size used in the present study. It is thus imperative to conduct a detailed study to further evaluate the potential of LAMP as a bedside diagnostic test for HAT.