著者

中村 寛治 宮地 有正

出版者

Japan Society of Civil Engineers

雑誌

環境工学研究論文集 (ISSN:13415115)

巻号頁・発行日

vol.29, pp.17-27, 1992-11-30 (Released:2010-03-17)

参考文献数

27

被引用文献数

4

---

A Phenol degrading bacterium, which could decompose TCE, was isolated and identified as Pseudomonas sp. KN1. The ability to degrade TCE was induced by addition of phenol. An enzyme responsible for TCE degradation was conjectured to be phenol hydroxylase (PH). A gene encoding PH was cloned from the chromosome DNA. Expression of the PH gene in Escherlchia coil DH5α was carried out by using pTrc100 as a vector. Although the gene cloned was expressed in the E. coil, phenol was oxidized only at a low rate. The degradation of TCE was not detected at all. A new plasmid vector, pRCL100, carrying the replicon of RSF1010 was constructed to use Pseudomonas sp.KN1 as a host. A higher activity of PH was observed when the PH gene was expressed under the control of the trc promoter in Pseudomonas sp. KN1. The TCE degradation was examined by using the genetically engineered Pseudomonas sp. KN1. TCE concentration in a vial was rapidly decreased when the bacterium engineered was inoculated, and the gene was expressed. The result showed that the gene encoding TCE degrading enzyme was the PH gene.