Processing and activation of the precursor of an extracellular <i>Streptomyces</i> transglutaminase were achieved by using three <i>Streptomyces</i> proteases (SAM-P20, SAM-P26 and SAM-P45), all of which are widely distributed in <i>Streptomyces</i>. The use of these proteases would allow us to develop a production process for the active form of this enzyme in recombinant bacteria.<br>