著者

Mamoru Oshiki Yuka Toyama Toshikazu Suenaga Akihiko Terada Yasuhiro Kasahara Takashi Yamaguchi Nobuo Araki

出版者

Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles

雑誌

Microbes and Environments (ISSN:13426311)

巻号頁・発行日

vol.37, no.2, pp.ME21090, 2022 (Released:2022-04-12)

参考文献数

61

被引用文献数

12

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Agricultural soil is the primary N2O sink limiting the emission of N2O gas into the atmosphere. Although Gemmatimonadetes bacteria are abundant in agricultural soils, limited information is currently available on N2O reduction by Gemmatimonadetes bacteria. Therefore, the effects of pH and temperature on N2O reduction activities and affinity constants for N2O reduction were examined by performing batch experiments using an isolate of Gemmatimonadetes bacteria, Gemmatimonas aurantiaca (NBRC100505T). G. aurantiaca reduced N2O at pH 5–9 and 4–50°C, with the highest activity being observed at pH 7 and 30°C. The affinity constant of G. aurantiaca cells for N2O was 4.4‍ ‍μM. The abundance and diversity of the Gemmatimonadetes 16S rRNA gene and nosZ encoding nitrous oxide reductase in agricultural soil samples were also investigated by quantitative PCR (qPCR) and amplicon sequencing ana­lyses. Four N2O-reducing agricultural soil samples were assessed, and the copy numbers of the Gemmatimonadetes 16S rRNA gene (clades G1 and G3), nosZ DNA, and nosZ mRNA were 8.62–9.65×108, 5.35–7.15×108, and 2.23–4.31×109 copies (g dry soil)–1, respectively. The abundance of the nosZ mRNA of Gemmatimonadetes bacteria and OTU91, OUT332, and OTU122 correlated with the N2O reduction rates of the soil samples tested, suggesting N2O reduction by Gemmatimonadetes bacteria. Gemmatimonadetes 16S rRNA gene reads affiliated with OTU4572 and OTU3759 were predominant among the soil samples examined, and these Gemmatimonadetes OTUs have been identified in various types of soil samples.

著者

Shohei Otomo Akihiko Terada Yu-You Li Kazuya Nishitoba Fumiaki Takakai Kunihiro Okano Naoyuki Miyata Shuhei Masuda

出版者

Japan Society on Water Environment

雑誌

Journal of Water and Environment Technology (ISSN:13482165)

巻号頁・発行日

vol.19, no.3, pp.139-152, 2021 (Released:2021-06-10)

参考文献数

54

被引用文献数

1

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In full-scale sewage treatment plants, long-term and high-frequency monitoring is required to mitigate nitrous oxide (N2O) emissions. In this study, the profile of the dissolved N2O concentration in a full-scale oxidation ditch reactor was investigated to determine the variation of the N2O emission factor. It was found that the concentration of dissolved N2O depended on microbial activity, which is affected by water temperature, dissolved oxygen concentration, and the dimensional relationship between the rotator and the inflow point. In the reactor, higher transcription levels of amoA mRNA and lower transcription levels of clade II type nosZ mRNA may be associated with N2O production. The emission factor for removed dissolved inorganic nitrogen presented a mean value of 0.86% and a median of 0.19%. When N2O production was promoted, gasification from the water surface was the most significant emission source, accounting for 52% of the total N2O emitted, on average. The N2O emission factor was often lower than 0.01% during stable operation; however, this factor was subject to sudden increases caused by nitrite accumulation.