- 著者
- Keiju Kishikawa Kenji Suetsugu Daisuke Kyogoku Kenji Ogaki Daisuke Iga Kohtaroh Shutoh Yuji Isagi Shingo Kaneko
- 出版者
- The Genetics Society of Japan
- 雑誌
- Genes & Genetic Systems (ISSN:13417568)
- 巻号頁・発行日
- vol.94, no.2, pp.95-98, 2019-04-01 (Released:2019-04-27)
- 参考文献数
- 16
- 被引用文献数
- 5 5
We developed microsatellite markers to compare the genetic variation between the putatively cleistogamous Gastrodia takeshimensis (Orchidaceae) and its chasmogamous sister species G. nipponica. We expected low genetic variation in G. takeshimensis in view of its hypothesized cleistogamy. Eighteen primer pairs were developed from a G. takeshimensis genomic DNA library, and their characteristics were tested for G. takeshimensis and G. nipponica. Seven loci were polymorphic in G. nipponica, whereas all loci showed no polymorphism in G. takeshimensis. Genetic diversity was thus not detected in G. takeshimensis, and it seems to have been lost by repeated selfing in the completely closed flower. The 18 markers described here will be useful for investigating the genetic variation between a cleistogamous species and its chasmogamous sister species.