- 著者
- Gillian L. Ryan Naoki Watanabe Dimitrios Vavylonis
- 出版者
- Japan Society for Cell Biology
- 雑誌
- Cell Structure and Function (ISSN:03867196)
- 巻号頁・発行日
- vol.38, no.1, pp.1-7, 2013 (Released:2013-01-19)
- 参考文献数
- 30
- 被引用文献数
- 5 12
We present a set of flexible image analysis tools to analyze dynamics of cell shape and protein concentrations near the leading edge of cells adhered to glass coverslips. Plugins for ImageJ streamline common analyses of microscopic images of cells, including the calculation of leading edge speeds, total and average intensities of fluorescent markers, and retrograde flow rate measurements of fluorescent single-molecule speckles. We also provide automated calculations of auto- and cross-correlation functions between velocity and intensity measurements. The application of the methods is illustrated on images of XTC cells.