著者

KAZUO IGARASHI FUMIYO KASUYA MIYOSHI FUKUI HISASHI NANJYOU

出版者

The Pharmaceutical Society of Japan

雑誌

Chemical and Pharmaceutical Bulletin (ISSN:00092363)

巻号頁・発行日

vol.35, no.7, pp.3033-3036, 1987-07-25 (Released:2009-10-19)

参考文献数

9

被引用文献数

4 5

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A high-performance liquid chromatographic (HPLC) method using a fluorescence detector is described for the simultaneous determination of dibucaine and its metabolites (M-4, M-8 and M-10) in human urine. Urine samples from obstetric patients were chromatographed in a reversed-phase system. When a ultraviolet detector (320 nm) was used, some interfering peaks appeared on the chromatogram, but this interference could be overcome by employing a fluorescence detector (Ex 330 nm and Em 440 nm) instead. The calibration curves were linear in the range of 0.05-5.0 μg/ml for all compounds and the detection limits of dibucaine and its metabolites were about 5 ng/ml in urine. The urinary excretion of dibucaine and its metabolites by obstetric patients infused with Percamine S® in the spinal cord were determined. The mean cumulative amounts of dibucaine, M-4, M-8 and M-10 excreted during 10 h after administration were 1.1, 10.5, 3.5 and 1.1 % of the dose, respectively. The total urinary excretion was 16.2% of the dose. This method is sufficiently sensitive and specific to permit the determination of dibucaine and its metabolites in biological fluids.