- 著者
- Hossain Md. Amran Ikeda Yutaka Hara Toru Nagasaki Yukio
- 出版者
- Elsevier
- 雑誌
- Colloids and surfaces. B, Biointerfaces (ISSN:09277765)
- 巻号頁・発行日
- vol.102, pp.778-782, 2013-02
- 被引用文献数
- 7 3
A new approach to the preparation of PEGylated [PEG: poly(ethylene glycol)] SiO2/Au hybrid nanoparticles was investigated. The synthesis of a PEGylated nanogel containing SiO2/Au hybrid nanoparticles was performed using matrix-catalyzed hydrolysis of tetraethyl orthosilicate, followed by the reduction of HAuCl4. UV–vis absorption of the prepared hybrid particles was obtained at 618 nm, which is a much longer wavelength than that of a nanogel containing only Au nanoparticles (523 nm). High-angle annular dark field images of the prepared particles observed using transmission electron microscopy and energy-dispersive X-ray spectroscopy confirmed the coexistence of Si and Au in the same particle. The presence of Si and Au in the prepared particles was also confirmed by inductively coupled plasma atomic emission spectroscopy. Dynamic light-scattering measurements of the particles in a highly ionic medium showed that they have high stability in both acidic and basic regions.
- 著者
- Toh Kazuko Yoshitomi Toru Ikeda Yutaka Nagasaki Yukio
- 出版者
- IOP PUBLISHING
- 雑誌
- Science and technology of advanced materials (ISSN:14686996)
- 巻号頁・発行日
- vol.12, no.6, pp.065001, 2011
- 被引用文献数
- 8
Gene therapy has generated worldwide attention as a new medical technology. While non-viral gene vectors are promising candidates as gene carriers, they have several issues such as toxicity and low transfection efficiency. We have hypothesized that the generation of reactive oxygen species (ROS) affects gene expression in polyplex supported gene delivery systems. The effect of ROS on the gene expression of polyplex was evaluated using a nitroxide radical-containing nanoparticle (RNP) as an ROS scavenger. When polyethyleneimine (PEI)/pGL3 or PEI alone was added to the HeLa cells, ROS levels increased significantly. In contrast, when (PEI)/pGL3 or PEI was added with RNP, the ROS levels were suppressed. The luciferase expression was increased by the treatment with RNP in a dose-dependent manner and the cellular uptake of pDNA was also increased. Inflammatory cytokines play an important role in ROS generation in vivo. In particular, tumor necrosis factor (TNF)-α caused intracellular ROS generation in HeLa cells and decreased gene expression. RNP treatment suppressed ROS production even in the presence of TNF-α and increased gene expression. This anti-inflammatory property of RNP suggests that it may be used as an effective adjuvant for non-viral gene delivery systems.