著者

JUN IIJIMA TOSHIHIRO YAMASE

出版者

The Society for Antibacterial and Antifungal Agents, Japan

雑誌

Journal of Microorganism Control (ISSN:27586383)

巻号頁・発行日

vol.28, no.3, pp.101-107, 2023 (Released:2023-10-23)

参考文献数

30

---

Abstract: Considering the lack of detailed research on the antibacterial mechanism of polyoxometalates, we examined the synergistic effect of novel bulky mixed Ti/W hetero-polyoxometalates (K9.5H2.5 [α-Ge2Ti4W20O78]・ 29H2O; αTi4, K9H5 [α-Ge2Ti6W18O77]・16H2O; αTi6, K23H5[α-Ge4Ti12W36O154]・39H2O; αTi12, K9H5 [β-Ge2Ti6W18O77]・ 45H2O; βTi6) with the antibiotic oxacillin against vancomycin intermediate-resistant Staphylococcus aureus (VISA) using fractional inhibitory concentration (FIC) index and growth curve in this study. All polyoxometalates used in this study showed remarkable synergistic effects with oxacillin. Its synergistic antibacterial mechanism was examined using reverse transcription PCR (RT-PCR) and penicillin binding protein-2’ (PBP2’) latex agglutination test. The results suggested that these polyoxometalates did not inhibit mecA gene transcription but resulted in PBP2’ protein malfunction. From these results, we concluded that the substances producing resistance in VISA were affected by polyoxometalates depending on their molecular size, facilitating a synergistic antibacterial effect with oxacillin.