著者

Jason E. SWAIN

出版者

THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT

雑誌

Journal of Reproduction and Development (ISSN:09168818)

巻号頁・発行日

vol.57, no.4, pp.551-555, 2011 (Released:2011-09-06)

参考文献数

29

被引用文献数

3 8

---

Elevated CO2 is required for in vitro embryo culture to maintain proper media pH and to supply embryo metabolic pathways. As an alternative to current approaches using gas cylinders, we examined use of a chemical reaction to supply CO2. A closed culture system was constructed and chemicals added to generate CO2, which was then supplied to developing embryos. This system was shown to provide a stable pH (7.2-7.4) over 4 days of use. One-cell mouse embryos were cultured in the device and no difference in blastocyst formation or cell number was apparent between embryos grown in a closed system with CO2 supplied by a chemical reaction or positive controls grown in a an open system in a CO2 incubator. This approach provides a highly purified, inexpensive, and easily obtainable gas source and offers potential for development of new, self-contained culture platforms.