著者

ITO-KAGAWA MIKIKO KOYAMA YASUO KONDO SHIGEJI

出版者

公益財団法人 日本感染症医薬品協会

雑誌

The Journal of Antibiotics

巻号頁・発行日

vol.37, no.5, pp.487-493, 1984

---

Formation of the basic antibiotic, K-582 was stimulated by supplying <i>Metarrhizium anisopliae</i> U-47 with several amino acids present in its structure. The addition of L-arginine to the basal medium resulted in the almost exclusive formation of K-582 B, while L-lysine increased K-582 A formation. Some carbon sources were observed to have effects similar to those obtained with the above mentioned amino acids. Furthermore, when L-arginine was added in excess to the basal medium, free &gamma;-hydroxyarginine, which is a major constituent of the antibiotic, accumulated extra- and intra-cellularly. Free &gamma;-hydroxyarginine isolated from the culture broth of this microorganism was the <i>threo</i>-L-isomer. K-582 formation was repressed by glycerol, which exerted catabolite repression of &gamma;-hydroxyarginine synthesis.