著者
Takahiro Noda Kaoru Daiou Takashi Mihara Yukio Nagano
出版者
Japanese Society of Breeding
雑誌
Breeding Science (ISSN:13447610)
巻号頁・発行日
pp.21021, (Released:2021-10-12)
被引用文献数
7

We previously developed insertion-deletion (InDel) markers that distinguish three genotypes (two homozygous and one heterozygous) of diverse citrus cultivars. These InDel markers were codominant and could be clearly detected by using simple agarose gel electrophoresis. We sought to establish a method for cultivar identification using these 28 InDel markers to genotype 31 citrus cultivars. The results revealed that a minimum of 6 markers were required to identify individuals using the three-genotype classification method. Furthermore, we found that a simple method for distinguishing between two genotypes (homozygous and heterozygous) could be used to identify individuals using a minimum of 7 markers. Our findings provide a basis for the development of simple and rapid citrus cultivar identification methods.