著者

Tsuyoshi Takamura Hisashi Suguro Yoshikazu Mikami Takashi Iwase Yusuke Komiyama Kayo Kuyama Kazuo Komiyama Hiderou Oki

出版者

日本大学歯学部

雑誌

Journal of Oral Science (ISSN:13434934)

巻号頁・発行日

vol.59, no.2, pp.279-287, 2017 (Released:2017-06-22)

参考文献数

28

被引用文献数

4

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Secretory leukocyte protease inhibitor (SLPI) is a serine protease inhibitor whose expression level is positively correlated with tumor aggressiveness and metastatic potential. However, the mechanism underlying SLPI-induced enhancement of malignant phenotype is not completely understood. The malignancy of cancer cells is highly dependent on cell migration activity. Our previous study revealed that gingival carcinoma Ca9-22 cells, but not colorectal adenocarcinoma HT-29 cells, expressed SLPI. Therefore, we investigated the migration activity of these two cell types to understand the nature of SLPI-mediated tumor aggressiveness and metastatic potential. In vitro wound healing assay indicated that HT-29 cells and SLPI-deleted Ca9-22 cells showed lower migration activity than wild-type Ca9-22 cells, suggesting that SLPI-induced cell migration plays an important role in tumor aggressiveness and metastatic potential. In addition, our gene expression profiling study based on microarray data for the three cell types identified a number of candidates, including LCP1 and GLI, that could be key molecules in the mechanism of SLPI-induced cell migration.

著者

Toshimitsu Iinuma Yasumichi Arai Midori Takayama Michiyo Takayama Yukiko Abe Yusuke Osawa Motoko Fukumoto Yusuke Fukui Yohei Shioda Nobuyoshi Hirose Kazuo Komiyama Nobuhito Gionhaku

出版者

日本大学歯学部

雑誌

Journal of Oral Science (ISSN:13434934)

巻号頁・発行日

vol.59, no.2, pp.207-213, 2017 (Released:2017-06-22)

参考文献数

24

被引用文献数

13

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Age-related deterioration in physical and oral health reduces healthy life expectancy and is thus an important problem for very elderly people. We investigated the effects of satisfaction with dietary life (SDL) in everyday life on oral health-related quality of life (OHRQoL) and subjective well-being and examined associations between these factors. We evaluated 426 elders aged 85 years or older. All participants completed a questionnaire that inquired about age, gender, drinking status, body mass index, cognitive function, disability, and comorbidities, among other covariates. Oral, physical, and mental health conditions were also examined. Associations of questionnaire results for SDL with items on subjective well-being (Philadelphia Geriatric Center Morale Scale [PGC] and World Health Organization-5 [WHO-5]) and OHRQoL (Geriatric Oral Health Assessment Index [GOHAI]) were confirmed with multiple logistic regression analysis. In a multivariate model adjusted for various confounders, participants with self-reported “enjoyable” SDL had significantly lower risks for having the lowest scores on the GOHAI, PGC, and WHO-5 (odds ratio [OR] = 0.460, 95% confidence interval [CI] = 0.277-0.762; OR = 0.589, 95% CI = 0.348-0.996; and OR = 0.452, 95% CI = 0.263-0.775, respectively). These associations remained after further adjustment for number of teeth.

著者

Taiichi Kitano Yoshikazu Mikami Takashi Iwase Masatake Asano Kazuo Komiyama

出版者

日本大学歯学部

雑誌

Journal of Oral Science (ISSN:13434934)

巻号頁・発行日

vol.58, no.2, pp.163-169, 2016 (Released:2016-06-25)

参考文献数

24

被引用文献数

10

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Porphyromonas gingivalis is important in the development of marginal periodontitis. However, the precise role and localization of P. gingivalis in chronic periapical periodontitis remain unclear. Thus, methods that can detect P. gingivalis in formalin-fixed and paraffin-embedded (FFPE) tissue samples are needed. We assessed a technique combining loop-mediated isothermal amplification (LAMP) with PCR (PCR-LAMP) for detection of P. gingivalis, using 110 FFPE tissue samples of chronic apical periodontitis. PCR-LAMP specifically detected P. gingivalis with high sensitivity in FFPE tissue samples, and the sensitivity of the technique was higher than that of PCR or LAMP alone. The results of immunohistochemistry (IHC) confirmed the specificity of PCR-LAMP. IHC showed that P. gingivalis was localized in a granular layer of chronic apical periodontitis, a region that correlated with the localization of macrophages. This is the first study to describe the localization of P. gingivalis in human periapical periodontitis. In conclusion, PCR-LAMP was an effective tool for detecting P. gingivalis in periapical periodontitis. In addition, IHC results improve our understanding of the role of P. gingivalis in the progression of periapical periodontitis. (J Oral Sci 58, 163-169, 2016)

著者

Hidero Ohki Mitsuhiko Matsumoto Mitsuharu Hasegawa Osamu Shimizu Shotaro Mukae Yusuke Amano Kazuo Komiyama

出版者

Nihon University School of Dentistry

雑誌

Journal of Oral Science (ISSN:13434934)

巻号頁・発行日

vol.47, no.4, pp.219-222, 2005 (Released:2006-01-17)

参考文献数

8

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A 54-year-old male presented with the complaint of a painful sore on the left side of his tongue. Our examination found an ulcer 15 × 20 mm in size on the left edge of the tongue, with peripheral indurations. The lesion was diagnosed histopathologically as squamous cell carcinoma (T2N0M0). Consequently, the lesion was surgical removed and radical neck dissection was performed. Four months after the operation, two unusual cyst-like lesions were identified in the parapharyngeal space by CT and MRI. A biopsy specimen revealed recurrent carcinoma with a cyst-like structure. The route of the tumor metastasis into the parapharyngeal space was obscured, but it was speculated that the excessive lymph accumulation was due to a lymphatic occlusion caused by the surgical procedure, proliferation of the metastatic carcinoma, or stagnation and accumulation of tissue fluid caused by parapharyngeal invasion by the recurrent lesion. (J. Oral Sci. 47, 219-222, 2005)