著者

LARROCHE CHRISTIAN GROS JEAN-BERNARD

出版者

公益社団法人日本生物工学会

雑誌

Journal of fermentation and bioengineering (ISSN:0922338X)

巻号頁・発行日

vol.74, no.5, pp.305-311, 1992-11-25

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The growth and sporulation of Penicillium roquefortii were studied during solid state cultivations on support. The process involved the use of a porous material, pozzolano, impregnated and continuously fed at a small rate by a concentrated liquid nutritive medium. This system allowed the recovery of the total spores and biomass produced, due to the large average radius, close to 5 μm, of the pores available for the fungal development. The sporulation occurred in two waves, the first beginning when nitrogen limitation appeared. Nitrogen balances showed that this first wave occurred with a sporulation efficiency, defined as the ratio of the nitrogen incorporated into the conidia to the nitrogen assimilated by the mycelium, close to unity. The second wave gave spores that did not contain nitrogen. Carbon balances, established using the stoichiometric equations of the metabolisms that occurred, showed that this second sporulation wave arose in connection with assimilatory growth that took place together with mecelial lysis. This system allowed 7×10^8 active spores/g pozzolano to be obtained, corresponding to 4.7×10^<11> spores/l fermentor at 110 h cultivation.