著者

Sandro GRILLI Giancarlo ARFELLINI Annamaria COLACCI Mario MAZZULLO Giorgio PRODI

出版者

The Japanese Cancer Association

雑誌

Japanese Journal of Cancer Research GANN (ISSN:09105050)

巻号頁・発行日

vol.76, no.8, pp.745-751, 1985 (Released:2008-03-17)

参考文献数

17

被引用文献数

1

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At 22hr after ip injection into male Wistar rats and BALB/c mice, chlorobenzene was covalently bound to DNA, RNA and proteins of the liver, kidney and lung, as has been found with various weak carcinogens. A microsome-mediated interaction with DNA occurred in vitro. The interaction was enhanced by pretreatment in vivo with phenobarbitone but was suppressed by addition of 2-diethylaminoethyl-2, 2-diphenylvalerate•HCl in vitro. These results indicate the involvement of cytochrome P-450. Liver microsomes were efficient bioactivators, whereas cytosol was ineffective. The extent of in vitro interaction of chlorobenzene with synthetic polyribonucleotides was of the same order as that with DNA. Finally, ultraviolet irradiation (λ=254nm or λmax=365nm) activated this environmental contaminant to forms capable of interacting with DNA. The results represent evidence for genotoxicity of chlorobenzene.