著者

Watcharapong TONPRASONG Masanao INOKOSHI Muneaki TAMURA Keita HATANO Shunsuke MINAKUCHI

出版者

The Japanese Society for Dental Materials and Devices

雑誌

Dental Materials Journal (ISSN:02874547)

巻号頁・発行日

vol.42, no.1, pp.49-54, 2023-01-25 (Released:2023-01-31)

参考文献数

31

被引用文献数

2

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Although a surface pre-reacted glass ionomer (S-PRG) exerts a suppressive effect on Candida albicans (C. albicans) activity and growth, its influence on the expression of the lipase gene (LIP) family including LIP1–LIP10, an indicator of clinical infection, has not yet been investigated. Therefore, in this study, we evaluated the effect of S-PRG filler eluates on LIP expression in C. albicans using real-time reverse-transcription polymerase chain reaction. Candida albicans was treated with an S-PRG filler diluted at ratios of 1:32 and 1:64 for 24 h at 37°C. The diluted S-PRG filler eluates (1:32) suppressed lipase activity in C. albicans by downregulating LIP5 (0.54±0.25 relative to that of the control) and LIP8 (0.35±0.074) expression after 24 h, which corresponded with decreased lipase activity. At a dilution factor of 1:64, there was no significant difference in LIP expression. Thus, the S-PRG filler eluate has potential to suppress fungal activity by downregulating LIP expression.