著者
Yutaka ORII Masayuki MORITA
出版者
The Japanese Biochemical Society
雑誌
The Journal of Biochemistry (ISSN:0021924X)
巻号頁・発行日
vol.81, no.1, pp.163-168, 1977-01-25 (Released:2008-11-18)
参考文献数
17

A method was established to estimate the pH change of several buffer solutions on freezing by using a combination of pH indicators. Among more than 30 buffer solutions examined, almost half exhibited a pH change in the temperature range between freezing point and 220°K; the results were tabulated. Glycerol was found to suppress the pH changes because of its “salt buffer” effect.