- 著者
- Masuda Tetsuya Taguchi Wakana Sano Ayane Ohta Keisuke Kitabatake Naofumi Tani Fumito
- 出版者
- Elsevier Masson SAS
- 雑誌
- Biochimie (ISSN:03009084)
- 巻号頁・発行日
- vol.95, no.7, pp.1502-1505, 2013-07
- 被引用文献数
- 30
Thaumatin, a sweet-tasting plant protein, elicits a sweet taste sensation at 50 nM in humans but not rodents. Although it was shown that the cysteine-rich domain (CRD) of human T1R3 (hT1R3) is important for the response to thaumatin, the amino acid residues within CRD critical for response are still unknown. A comparison of the amino acid sequence (69 amino acid residues) of CRD between hT1R3 and mouse T1R3 (mT1R3) revealed sixteen amino acids that differ. In the present study, we converted each of these sixteen amino acids in hT1R3 to their mouse counterpart and examined the response to thaumatin and sucralose using a cell-based assay. No significant decrease in the response to sucralose was seen among any of the sixteen mutants. However, five mutants (Q504K, A537T, R556P, S559P, and R560K) exhibited a significantly diminished response to thaumatin. The five critical residues involved in the response to thaumatin were dispersed in the CRD of hT1R3 and widely distributed when compared to brazzein. The unique intense sweet-taste of thaumatin might be attributed to the different receptor activation mechanism compared to the small molecule sweetener sucralose.
- 著者
- Sugahara Michihiro Song Changyong Suzuki Mamoru Masuda Tetsuya Inoue Shigeyuki Nakane Takanori Yumoto Fumiaki Nango Eriko Tanaka Rie Tono Kensuke Joti Yasumasa Kameshima Takashi Hatsui Takaki Yabashi Makina Nureki Osamu Numata Keiji Iwata So
- 出版者
- Nature Publishing Group
- 雑誌
- Scientific reports (ISSN:20452322)
- 巻号頁・発行日
- vol.6, 2016-04-18
- 被引用文献数
- 45
結晶を損傷しない新しいタンパク質結晶の輸送媒体を発見-タンパク質の結晶構造解析で新薬創生に一歩近づく. 京都大学プレスリリース. 2016-04-22.