- 著者
- Akira ISHIHAMA Masayoshi ENAMI Yoshikazu NISHIJIMA Toshikazu FUKUI Eiko OHTSUKA Morio IKEHARA
- 出版者
- The Japanese Biochemical Society
- 雑誌
- The Journal of Biochemistry (ISSN:0021924X)
- 巻号頁・発行日
- vol.87, no.3, pp.825-830, 1980-03-01 (Released:2008-11-18)
- 参考文献数
- 23
The effects of 2'-substitutions of ATP on the substrate and inhibitor properties for RNA synthesis were studied in the poly(dAT)-dependent reaction of Escherichia coli RNA polymerase. In the presence of UTP, 2'-deoxy-2'-azidoadenosine 5'-triphosphate (AzTP) was incorporated into an acid-insoluble fraction at one-tenth of the rate of ATP incorporation; it thus acts as a competitive inhibitor for poly(AU) synthesis. On the other hand, another ATP analog, 2'-deoxy-2'-fluoroadenosine 5'-triphosphate (AfTP), was co-polymerized with UTP into acid-insoluble materials at a rate less than 1% of that of ATP incorporation; in addition, it exerted a strong but mixed-type inhibition on poly(AU) synthesis. Different modes of action of the two ATP analogs are discussed in connection with the specificity of substrate recognition by RNA polymerase.
- 著者
- AKIHIRO YAMAZAKI TERUO FURUKAWA MASAO AKIYAMA MASARU OKUTSU IZUMI KUMASHIRO MORIO IKEHARA
- 出版者
- The Pharmaceutical Society of Japan
- 雑誌
- Chemical and Pharmaceutical Bulletin (ISSN:00092363)
- 巻号頁・発行日
- vol.21, no.4, pp.692-696, 1973-04-25 (Released:2011-02-08)
- 参考文献数
- 17
- 被引用文献数
- 3 7
As the analog of thioinosine, 2-methyl-, 2-ethyl-, 2-methylthio-, and 2-methyiamino-6-mercapto-9-β-D-ribofuranosylpurine were synthesized. From thio-AICA-riboside, 5-formamido-1-(2', 3', 5'-tri-O-formyl-β-D-ribofuranosyl)-4-imidazolethiocarboxamide and 5-acetamido-1-(2', 3', 5'-tri-O-acetyl-β-D-ribofuranosyl)-4-imidazolethiocarboxamide were also prepared.