著者

Suzuki Hitoshi Iwasa Masahiro A. Ishii Nobuo NAGAOKA Hiroko TSUCHIYA Kimiyuki

出版者

日本哺乳類学会

雑誌

Mammal study = The Continuation of the Journal of the Mammalogical Society of Japan (ISSN:13434152)

巻号頁・発行日

vol.24, no.1, pp.43-50, 1999-06-01

被引用文献数

6 11

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We examined the geographic variation of <i>Tokudaia osimensis</i> through the analysis of mitochondria1 cytochrome <i>b</i> (cyt <i>b</i>) gene sequences and the restriction fragment length polymorphism (RFLP) in the nuclear ribosomal RNA gene (rDNA), using samples collected from Tokuno-shima and Amami-oshima in the Ryukyu Islands. The two populations show intrinsic karyological variation (Tokuno-shima, 2n=45; Amami-oshima, 2n=25). Sequences of the cyt <i>b</i> gene differed considerably between the two island populations. The extent of the sequence divergence among 1,140 bp of the gene was calculated to be 0.088 using the Kimura two parameter method, and was comparable to those between related species of rodents such as within genus <i>Mus</i> or <i>Rattus</i>. The extent of the differentiation in the rDNA-RFLP was also high. Three out of 22 restriction site variants were found to be fixed in the nuclear rDNA arrays of hundreds of copies in either one of the two island populations. These intensive inter-populational differences indicate that the two island populations may have been isolated for a considerable period of evolutionary time, probably several millions of years, despite there having been several opportunities for renewed genetic contact during the Pleistocene ice ages. Our data strongly suggest that the current taxonomic status of the populations of the two islands, Amami-oshima and Tokuno-shima, which regards them conspecific, should be reviewed.