著者

Mikio Furuse Daiki Nakatsu Wendy Hempstock Shiori Sugioka Noriko Ishizuka Kyoko Furuse Taichi Sugawara Yugo Fukazawa Hisayoshi Hayashi

出版者

Japan Society for Cell Biology

雑誌

Cell Structure and Function (ISSN:03867196)

巻号頁・発行日

pp.22068, (Released:2022-12-09)

被引用文献数

6

---

The claudin family of membrane proteins is responsible for the backbone structure and function of tight junctions (TJs), which regulate the paracellular permeability of epithelia. It is thought that each claudin subtype has its own unique function and the combination of expressed subtypes determines the permeability property of each epithelium. However, many issues remain unsolved in regard to claudin functions, including the detailed functional differences between claudin subtypes and the effect of the combinations of specific claudin subtypes on the structure and function of TJs. To address these issues, it would be useful to have a way of reconstituting TJs containing only the claudin subtype(s) of interest in epithelial cells. In this study, we attempted to reconstitute TJs of individual claudin subtypes in TJ-deficient MDCK cells, designated as claudin quinKO cells, which were previously established from MDCK II cells by deleting the genes of claudin-1, -2, -3, -4, and -7. Exogenous expression of each of claudin-1, -2, -3, -4, and -7 in claudin quinKO cells resulted in the reconstitution of functional TJs. These TJs did not contain claudin-12 and -16, which are endogenously expressed in claudin quinKO cells. Furthermore, overexpression of neither claudin-12 nor claudin-16 resulted in the reconstitution of TJs, demonstrating the existence of claudin subtypes lacking TJ-forming activity in epithelial cells. Exogenous expressions of the channel-forming claudin-2, -10a, -10b, and -15 reconstituted TJs with reported paracellular channel properties, demonstrating that these claudin subtypes form paracellular channels by themselves without interaction with other subtypes. Thus, the reconstitution of TJs in claudin quinKO cells is advantageous for further investigations of claudin functions.Key words: Tight junction, Claudin, Paracellular permeability, Epithelial barrier

著者

石塚 則子 Noriko Ishizuka

出版者

同志社大学人文学会

雑誌

同志社大学英語英文学研究 = Doshisha studies in English (ISSN:02861291)

巻号頁・発行日

no.102, pp.i-ix, 2021-03

---

林以知郎先生 Leo J. Loveday先生 御退職記念号