著者

Tsai Melody Ohniwa Ryosuke L Kato Yusuke Takeshita Sayaka L Ohta Toshiko Saito Shinji Hayashi Hideo Morikawa Kazuya

出版者

BioMed Central

雑誌

BMC microbiology (ISSN:14712180)

巻号頁・発行日

vol.11, pp.13, 2011-01

被引用文献数

100 26

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BackgroundThe ability of staphylococci to grow in a wide range of salt concentrations is well documented. In this study, we aimed to clarify the role of cardiolipin (CL) in the adaptation of Staphylococcus aureus to high salinity.ResultsUsing an improved extraction method, the analysis of phospholipid composition suggested that CL levels increased slightly toward stationary phase, but that this was not induced by high salinity. Deletion of the two CL synthase genes, SA1155 (cls1) and SA1891 (cls2), abolished CL synthesis. The cls2 gene encoded the dominant CL synthase. In a cls2 deletion mutant, Cls1 functioned under stress conditions, including high salinity. Using these mutants, CL was shown to be unnecessary for growth in either basal or high-salt conditions, but it was critical for prolonged survival in high-salt conditions and for generation of the L-form.ConclusionsCL is not essential for S. aureus growth under conditions of high salinity, but is necessary for survival under prolonged high-salt stress and for the generation of L-form variants.

著者

ZAITSU Takashi OHTA Toshiko OHSHIMA Hiroshi MUKAI Chiaki

出版者

THE JAPAN SOCIETY FOR AERONAUTICAL AND SPACE SCIENCES

雑誌

TRANSACTIONS OF THE JAPAN SOCIETY FOR AERONAUTICAL AND SPACE SCIENCES, AEROSPACE TECHNOLOGY JAPAN (ISSN:18840485)

巻号頁・発行日

vol.12, no.29, pp.Tp_7-Tp_9, 2014

被引用文献数

1

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Occurrences of dental/oral diseases have been reported in the space environment and pre- or post-spaceflight and the deteriorations of oral defensive functions are evident in the space environment simulated experiments. NASA has identified 6 dental problems (Abscess, Avulsion/Tooth Loss, Caries, Crown Replacement, Exposed Pulp/Pulpitis and Filling Replacement) as the most important dental incidents to be dealt with in the long-term mission. To focus on the dental/oral health management for astronauts, dental examination and dental orthopantomogram are conducted as dental diagnosis. In addition, dental nerve block, dental prosthesis replacement and dental pain control are described in the treatment manual on the International Space Station (ISS). However, the systematic program to evaluate the risk of dental/oral problems in spacecraft and the manual to prevent the dental/oral problems in the space environment are still far from sufficient. To promote the dental/oral health of astronauts, the authors are going to launch "Space Oral Health Promotion (SOHP) project" which will tackle the research tasks and the operational countermeasures in space dentistry.