著者

SAKUMA FUTOSHI

出版者

The Japanese Biochemical Society

雑誌

The Journal of Biochemistry (ISSN:0021924X)

巻号頁・発行日

vol.12, no.2, pp.247-279, 1930

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1. The distribution of glyoxalase in animal tissues was ex-amined. Generally speaking, the glyoxalase content was highest in the liver, the other tissues containing 30-60% of that of the liver. There was no noticeable fluctuation in the glyoxalase distribution in the tissues, so long as the animal was kept under normal conditions. The main difference between warm- and cold-blooded animals was that the liver of the latter was 50% inferior to that of the former in its glyoxalase content.<br> 2. The glvoxalase contents of the animal tissues decreased definitely when the tissues were subjected to continuous deprivation of sugar. The liver lost the enzyme most slowly in comparison with other tissues.<br> 3. The relation between the glyoxalase content and the process of germination of the soy-bean was studied. Glyoxalase of the bud increased rapidly up to the 7th or 8th clay of germinationn, and was succeeded by a subsequent declination. This increase in gly oxalase may be attributed to the increase of co-enzyme rather than to that of the enzyme itself.<br> 4. The dry glyoxalase sample was prepared by the successive treatment of animal tissues with alcohol and ether. The powder retained 70% activity of the original fresh tissue and could be kept for at. least four weeks without' loss of its power. The extraction of the enzyme from the powder was achieved most effectively by shaking it with a neutral liquid for two hours at room temperature or for an hour at 37°.<br> 5. When glyoxalase was dialysed through collodion membrane against distilled water, its activity disappeared after 5 hours. The lost activity was restored definitely by the addition of boiled tissue juice.<br> 6. Glucose and polysaccharides, which contain glucose in the molecules, accelerated the activity of dialysed and non-dialysed glyoxayase. The hexoses other than glucose were quite indifferent to the enzyme. Inorganic phosphate inhibited the glyoxalase action. Guanine and its derivatives reacted directly with methylglyoxal. When mnet.hlyglyoxal was brought into contact with amino acids, rather a rapid disappearance of methylglyoxal was observed, coupled with the production of NH₃ and CO₂. The amount of liberated NH₃ and CO₂ was 70-80% of the value computed from the quantity of amino acids involved in the reaction.<br> 7. Fosters pancreatic powder which, according to her statement, inhibits the lactic acid formation in chopped muscle and differs from "antiglyoxalase" in some respects, seems to be identical with "antiglyoxalase" in every point.