- 著者
- Ayumi MUKUNOKI Toru TAKEO Satohiro NAKAO Kana TAMURA Yuka HORIKOSHI Naomi NAKAGATA
- 出版者
- Japanese Association for Laboratory Animal Science
- 雑誌
- Experimental Animals (ISSN:13411357)
- 巻号頁・発行日
- pp.20-0042, (Released:2020-06-17)
- 被引用文献数
- 2
The cold storage of two-cell embryos is a useful technique for transporting genetically engineered mice without the shipment of live animals. However, the developmental ability of cold-stored embryos decreases with prolonged storage periods. Therefore, the transported embryos must be readily transferred to recipient mice upon arrival. The cryopreservation of cold-transported embryos may improve the flexibility of the schedule of embryo transfer. In this paper, we examined the viability and developmental ability of vitrified-warmed mouse embryos at the two-cell stage after cold storage in refrigerated temperatures for 0, 24, 48, 72, or 96 hours. The viability of vitrified-warmed embryos after cold storage was comparable to vitrified-warmed embryos without cold storage. Vitrified-warmed embryos after cold storage also developed normally to pups by embryo transfer. In addition, live pups were obtained from vitrified-warmed embryos after cold-transportation from Asahikawa Medical University. In summary, cold-stored embryos can be used for the transportation and archive of genetically engineered mice.
- 著者
- Ayumi MUKUNOKI Toru TAKEO Satohiro NAKAO Kana TAMURA Yuka HORIKOSHI Naomi NAKAGATA
- 出版者
- Japanese Association for Laboratory Animal Science
- 雑誌
- Experimental Animals (ISSN:13411357)
- 巻号頁・発行日
- vol.69, no.4, pp.423-429, 2020 (Released:2020-11-12)
- 参考文献数
- 23
- 被引用文献数
- 1 2
The cold storage of two-cell embryos is a useful technique for transporting genetically engineered mice without the shipment of live animals. However, the developmental ability of cold-stored embryos decreases with prolonged storage periods. Therefore, the transported embryos must be readily transferred to recipient mice upon arrival. The cryopreservation of cold-transported embryos may improve the flexibility of the schedule of embryo transfer. In this paper, we examined the viability and developmental ability of vitrified-warmed mouse embryos at the two-cell stage after cold storage in refrigerated temperatures for 0, 24, 48, 72, or 96 h. The viability of vitrified-warmed embryos after cold storage was comparable to vitrified-warmed embryos without cold storage. Vitrified-warmed embryos after cold storage also developed normally to pups by embryo transfer. In addition, live pups were obtained from vitrified-warmed embryos after cold-transportation from Asahikawa Medical University. In summary, cold-stored embryos can be used for the transportation and archive of genetically engineered mice.