- 著者
- Seif-Eldin Nasr Ayyad Khalid Omar Al-Footy Walied Mohamed Alarif Tariq Rashad Sobahi Salim Ahmed Bassaif Mohamed Saleh Makki Abdullah Mohamed Asiri Amani Yahya Al Halawani Adel Farid Badria Farid Abd Al-raheem Badria
- 出版者
- The Pharmaceutical Society of Japan
- 雑誌
- Chemical and Pharmaceutical Bulletin (ISSN:00092363)
- 巻号頁・発行日
- vol.59, no.10, pp.1294-1298, 2011-10-01 (Released:2011-10-01)
- 参考文献数
- 16
- 被引用文献数
- 17 38
The petroleum ether extract of the red alga Laurencia obtusa afforded three new C15 acetogenins (cyclic ether enyne): (12Z)-cis-maneonene-D (1), (12E)-cis-maneonene-E (2), and (12Z)-trans-maneonene-C (3), along with one known cis-maneonene-A (4). Blood neutrophils were prepared, cultured, and incubated for 24, 48, and 72 h in medium with and without isolated compounds. Blood neutrophils were prepared, cultured, and incubated for 24, 48 and 72 h in medium with and without the isolated compounds. Both morphology and DNA fragmentation methods assessed the percentage of neutrophils apoptosis in each culture. In the present study, several observations have been made concerning the apoptosis-inducing or inhibiting effect of 1 and 2. Both compounds had no inhibition of apoptosis but apoptosis was enhanced significantly by aging. However, 1 stimulated apoptosis of normal only at the initial 24 h. After that there was no significant difference in apoptosis with or without compound 1, while 2 stimulated apoptosis at all the times. The apoptosis induced by these two compounds was demonstrated by DNA fragmentation assay and microscopic observation. These observations suggest that compounds 1 and 2 may be involved in regulation of programmed death in the initiation and propagation of inflammatory responses.