- 著者
- Ijiri Shigeho Shibata Yasushi Takezawa Nonoha Kazeto Yukinori Takatsuka Naoki Kato Erika Hagihara Seishi Ozaki Yuichi Adachi Shinji Yamauchi Kohei Nagahama Yoshitaka
- 出版者
- Endocrine Society
- 雑誌
- Endocrinology (ISSN:00137227)
- 巻号頁・発行日
- vol.158, no.3, pp.627-639, 2017-03
- 被引用文献数
- 3
The maturation-inducing hormone 17(alpha), 20(beta)-dihydroxy-4-pregnen-3-one (DHP) was first identified in the amago salmon. Although carbonyl reductase-like 20 beta-hydroxysteroid dehydrogenase (CR/20b-HSD) was reported to convert 17 alpha-hydroxyprogesterone (17OHP) to DHP in rainbow trout, we previously found that CR/20 beta-HSD messenger RNA (mRNA) was not upregulated in stimulated granulosa cells from masu salmon, which suggested that DHP is synthesized by a different enzyme. Accordingly, the current study aimed to identify the specific 20 beta-hydroxysteroid dehydrogenase (20 beta-HSD) responsible for DHP production by granulosa cells during final oocyte maturation in masu salmon. RNA sequencing was performed on granulosa layers that were isolated from ovarian follicles at 1 month before ovulation and incubated with or without forskolin, which was used to mimic luteinizing hormone, and similar to 12 million reads were obtained, which yielded 71,062 contigs of > 100 bp. tBlastx analysis identified 1 contig (#f103496)as similar to 17 beta-hydroxysteroid dehydrogenase type 12 (hsd17 beta 12); however, because the full-length #f103496 sequence was different from hsd17 beta 12, it was termed hsd17 beta 12-like (hsd17 beta 12l). We found that mammalian cells transfected with full-length hsd171 beta 2l exhibited considerable 20b-HSD activity, as indicated by efficient conversion of exogenous 17OHP to DHP. In addition, we found that hsd17 beta 12l mRNA levels were consistently low in follicles during vitellogenic growth; however, the levels increased significantly during final oocytematuration. The levels of hsd17 beta 12lmRNA were also considerably increased in granulosa layers in which 20 beta-HSD activity was induced by salmon pituitary extract. Therefore, we suggest that hsd17 beta 12l, not CR/20 beta-HSD, is the 20 beta-HSD responsible for DHP production by granulosa cells in masu salmon during final oocytematuration.
- 著者
- Shibata Yasushi
- 出版者
- ASEAN Neurological Association
- 雑誌
- Neurology Asia (ISSN:18236138)
- 巻号頁・発行日
- vol.18, no.4, pp.413-414, 2013-12
This is the report of a patient with cluster headaches preceded by back pain. The patient's back pain was provoked by arm and shoulder movement. Mild back pressure caused severe back pain and, as a result, he could not lie in a supine position. His back pain was usually mild, however he sometimes experienced severe stabbing and aching pain. We diagnosed the back pain as allodynia. We Herein discuss the diagnosis of back pain in this case and its relationship with cluster headaches.