著者

伊藤 慈晃 イトウ シゲアキ Shigeaki Ito

出版者

立教大学大学院社会学研究科

雑誌

社会学研究科年報 (ISSN:13466259)

巻号頁・発行日

no.25, pp.75-79, 2018

著者

伊藤 慈晃 イトウ シゲアキ Shigeaki Ito

出版者

立教大学大学院社会学研究科

雑誌

社会学研究科年報 (ISSN:13466259)

巻号頁・発行日

no.27, pp.19-30, 2020

著者

伊藤 慈晃 イトウ シゲアキ Shigeaki Ito

雑誌

社会学研究科年報

巻号頁・発行日

vol.25, pp.75-79, 2018

著者

Yuichiro Takanami Nobumasa Kitamura Shigeaki Ito

出版者

The Japanese Society of Toxicology

雑誌

The Journal of Toxicological Sciences (ISSN:03881350)

巻号頁・発行日

vol.45, no.12, pp.769-782, 2020 (Released:2020-12-01)

参考文献数

25

被引用文献数

2

---

A novel tobacco vapor product (NTV) contains tobacco leaves and generates nicotine-containing aerosols using heating elements. Subchronic biological effects have been evaluated previously using three-dimensional bronchial epithelial model cells by repeated exposure to cigarette smoke (CS) and the NTV aerosols; however, the intracellular exposure characteristics have not been studied in detail. In this study, cells were initially exposed to an aqueous extract (AqE) of cigarette smoke (CS) at two concentration levels, and the cell lysate underwent untargeted analysis by LC-high resolution mass spectrometry to determine the exogenous compounds present in the cells. Among the thousands of peaks detected, four peaks showed a CS-dependency, which were reproducibly detected. Two of the peaks were nicotine and nicotine N-oxide, and the other two putative compounds were myosmine and norharman. The cells were then exposed to an AqE of CS in various combinations of exposure and post-exposure culture durations. Post-exposure culturing of cells with fresh medium markedly decreased the peak areas of the four compounds. The in-vitro switching study of CS to NTV aerosols was investigated by intermittently exposing cells to an AqE of CS four times, followed by exposure to either an AqE of CS, NTV aerosol or medium another four times. Switching to NTV reduced myosmine and norharman levels, which are known CS constituents. The results indicate that extracellular compounds inside cells reflect the exposure state outside cells. Thus, monitoring functional changes to cells in these exposure experiments is feasible.